产品说明
包装
1250 units in poly bottle
应用
在 131 I 离子标记研究、蛋白质溴化和 36 Cl 标记大分子的长期分离程序中，可作为乳过氧化物酶的有效替代品。
生化/生理作用
Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H2O2 decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).
单位定义
One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 ℃ in the presence of potassium chloride and H2O2.
外形
Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5
基本信息
| 酶学委员会(EC)编号 | 1.11.1.10 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00130774 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 形式 | buffered aqueous suspension |
| specific activity | 1,000-2,000 units/mg protein (E |
| 分子量 | 42 kDa |
| 吸光度比值 | RZ ~1.0 |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
