The scope of this study is to make evaluations on faunistic, ecological and zoogeographical composition of the subfamily Aleocharinae (Coleoptera: Staphylinidae) of the Central Anatolian Region of Turkey. Ninety-three species, belonging to 26 genera and 11 tribes, were detected by field studies between years 2009–2011 in the Central Anatolian Region. It is concluded that among these, one species from Atheta (Atheta camardiensis, Sert et al., 2013) and Gyrophaena (Gyrophaena cagatay, Sert et al., 2013) respectively are new to science and seven species from Atheta, Brachyusa, Ischnopoda and Liogluta are new genera records for Turkey (Sert et al., 2013a). Eight species are endemic for Turkey and 69 species are new records for Central Anatolian Region.