Clonostachys rosea, Clonostachys rosea liquid and application of Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary

[0027] Embodiment 1: Preparation of Gliocus pink MES72701 bacterial liquid

[0028] This embodiment provides a method for preparing a Gliocladium rosea MES72701 bacterial liquid, which includes the following steps.

[0029] 1. Inoculation and cultivation

[0030] Pick the fresh mycelium of MES72701 that has been activated and cultured, inoculate it on solid PDA medium, inoculate 3 plates, and then place it in a constant temperature incubator at 28°C for 15 days. The formula of PDA medium is: glucose 20g / L, potato 200g / L, agar 19g / L, water 1000mL, natural pH; the configuration method of PDA medium refers to the preparation of conventional potato glucose medium.

[0031] 2. Preparation of spore solution

[0032] After 15 days, 25 mL of sterile water (containing 0.05% Tween 80) was added to each petri dish, the spores were washed with a spreader, filtered with four layers of gauze, and a 10-fold gradient dilution was performed.

[0033] 3. Detection of spore content

[0034] ...

[0035] Embodiment 2: Bacteriostasis test of Glioma pink MES72701 bacterium liquid to sunflower sclerotinia

[0036] 1. Test time and place: In December 2019, it will be carried out in the laboratory of the company's R&D center.

[0037] 2. Test method:

[0038] (1) The source of the tested bacteria

[0039] Sclerotinia sclerotiorum was isolated from the sunflower sclerotiorum pathogenic field in Chifeng City, Inner Mongolia, cultivated and purified by the R&D personnel of the company, and finally identified as Sclerotinia sclerotiorum (Lib.) deBary by 16S rDNA, and identified as Sclerotinia sclerotiorum (Lib.) deBary by pathogenicity assay Strong pathogenicity.

[0040] Glioma pink fungus liquid is obtained by inoculating, culturing, and preparing spore liquid from the bacterial species described in Example 1.

[0041] (2) Plate antagonism experiment

[0042] To prepare a PDA solid plate, pipette 200 μL of the spore suspension prepared in Example 1 and apply it evenly on t...

[0046] Embodiment 3: Potted control effect of Glioma pink MES72701 bacterium liquid to sunflower sclerotinia

[0047] 1. Test drug

[0048] (1) Microbial agent

[0049] Glioma pink MES72701 bacterium liquid (MES72701 spore suspension) prepared in embodiment 1, spore content is 2 * 10 7 individual / mL.

[0050] (2) Chemical agents

[0051] 50% procymidone wettable powder 2000 times liquid (Shandong Haixun Biochemical Co., Ltd.).

[0052] (3) Blank control

[0053] Set clean water treatment as blank control.

[0054] 2. Test time and place

[0055] The test was carried out in the company's nursery in December 2019.

[0056] 3. Test method

[0057] Treatment settings: the sunflower variety is Longshikui No. 2, and the test set 5 treatments, which are respectively the original concentration of the spore suspension described in Example 1, dilution 1 times, dilution 3 times, dilution 5 times and adding water without adding bacteria control , 50% procymidone wettable powder 2...