Application of AOL-S00006g439 gene in regulation and control of arthrobotrys oligospora production trap

A technology of Arthrobuspora oligosporum, gene, applied in the field of microbiology

Active Publication Date: 2022-07-22
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the existing research is to induce the production of predators by exogenous induction meth

Method used

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  • Application of AOL-S00006g439 gene in regulation and control of arthrobotrys oligospora production trap
  • Application of AOL-S00006g439 gene in regulation and control of arthrobotrys oligospora production trap
  • Application of AOL-S00006g439 gene in regulation and control of arthrobotrys oligospora production trap

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Experimental program
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Embodiment

[0020] 1. Materials and methods

[0021] 1. Culture medium

[0022] The mass ratio of WA medium: agar is 15%, and the balance is deionized water.

[0023] CMY medium: boil 30g corn kernels in boiling water for 30min, filter with gauze, add 7g yeast powder, 15g agar, deionized water to 1000mL, sterilize for later use.

[0024] WA medium was used for the culture and trap observation of Arthropoda oligospora wild-type Wide-type and knockout strain ΔAoNpr1; CMY medium was used for the culture of wild-type Wide-type and knockout strain ΔAoNpr1 spores.

[0025] 2. Experimental strains and nematodes

[0026] The wild-type YMF1.01883 of Arthropoda oligosporum used in this experiment is stored in the Wild Organism Germplasm Resource Bank of Southwest China-Yunnan University Microorganism Branch, and the knockout strain ΔAoNpr1 is the strain obtained by knocking out the Npr1 gene of Arthropoda oligosporum in our research group; The nematodes used in the experiments were holotooth rea...

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Abstract

The invention relates to the technical field of microorganisms, in particular to application of an AOL-S00006g439 gene to regulation and control of an arthrobotrys oligospora production trap. The AOL-S00006g439 gene in the arthrobotrys oligospora can influence the formation of the trap for the first time, after the gene is knocked out, the arthrobotrys oligospora does not need to be induced by nematodes, ascaroside, ammonia, urea and the like, a large number of traps can be spontaneously produced, the arthrobotrys oligospora can effectively prey the nematodes, the arthrobotrys oligospora can be transformed through the gene engineering means, the spontaneous production of the traps becomes possible, and the arthrobotrys oligospora can be used for the first time. An efficient biocontrol strain is provided for agricultural nematode diseases.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of AOL-S00006g439 gene in regulating and controlling Arthrospora oligospora producing traps. Background technique [0002] Plant-parasitic nematodes cause huge threats and economic losses to the security of global food crops every year. The use of nematode chemical control has gradually decreased due to the easy cause of drug resistance, chemical residues of agricultural and livestock products, and ecological environment damage. Therefore, nematode biological control has received widespread attention from the society. In nature, nematode-predating fungi mainly produce predators under the stimulation of nematodes and compounds such as ascarosides, ammonia, and urea, and play a role in predation. [0003] Arthrobotrys oligospora is a type species of nematode-predating fungi. It has been reported that amino acids, polypeptides, ascarosides, ammonia, and urea ...

Claims

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Application Information

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IPC IPC(8): C12N15/31C12N1/15C12N15/80C12R1/645
CPCC07K14/37C12N15/80
Inventor 王芯郑喜田梦青张克勤
Owner YUNNAN UNIV
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