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Semicontinuous and Continuous Production of Chloroperoxidase by Caldariomyces fumago Immobilized in k-Carrageenan.

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  • 1. Department of Microbiology, University of Alberta, and Alberta Research Council, Edmonton, Alberta, Canada T6G 2E9.
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    • Carmichael RD 1
    (1 author)

Applied and Environmental Microbiology, 01 Feb 1986, 51(2):276-280
https://doi.org/10.1128/aem.51.2.276-280.1986 PMID: 16346984 PMCID: PMC238859

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Abstract 

Three strains of Caldariomyces fumago were immobilized in 4% k-carrageenan and tested for semicontinuous production of chloroperoxidase (CPO). Over an 80-day period, growing in defined medium, C. fumago strains CMI 89362 and ATCC 11925 produced enzyme concentrations of 99 and 71 mg/liter, respectively, during six production periods of 12 to 14 days, while C. fumago DAOM 137632 produced only 24 mg of CPO per liter during six growth periods of 10 days. CPO production was unaffected by various regimens of washing between transfers. Mycelial growth was primarily restricted to the head surface, and bead size increased linearly with time. Attempts to restrict growth but maintain CPO production were unsuccessful. Pigment production, fructose utilization, and pH change in the immobilized cell cultures compared closely with the growth characteristics of free cell cultures. By using an airlift tower fermentor with an external loop run with continuous medium replacement of 20 ml/h (D = 0.016), strain CMI 89362 in bead form produced CPO at 40 mg/liter for 11 days.

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Appl Environ Microbiol. 1986 Feb; 51(2): 276–280.
PMCID: PMC238859
PMID: 16346984

Semicontinuous and Continuous Production of Chloroperoxidase by Caldariomyces fumago Immobilized in k-Carrageenan

Robert D. Carmichael,1 Alan Jones,2 and Michael A. Pickard1,*
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Abstract

Three strains of Caldariomyces fumago were immobilized in 4% k-carrageenan and tested for semicontinuous production of chloroperoxidase (CPO). Over an 80-day period, growing in defined medium, C. fumago strains CMI 89362 and ATCC 11925 produced enzyme concentrations of 99 and 71 mg/liter, respectively, during six production periods of 12 to 14 days, while C. fumago DAOM 137632 produced only 24 mg of CPO per liter during six growth periods of 10 days. CPO production was unaffected by various regimens of washing between transfers. Mycelial growth was primarily restricted to the head surface, and bead size increased linearly with time. Attempts to restrict growth but maintain CPO production were unsuccessful. Pigment production, fructose utilization, and pH change in the immobilized cell cultures compared closely with the growth characteristics of free cell cultures. By using an airlift tower fermentor with an external loop run with continuous medium replacement of 20 ml/h (D = 0.016), strain CMI 89362 in bead form produced CPO at 40 mg/liter for 11 days.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Chibata I, Tosa T. Use of immobilized cells. Annu Rev Biophys Bioeng. 1981;10:197–216. [Abstract] [Google Scholar]
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  • Sato T, Nishida Y, Tosa T, Chibata I. Immobilization of Escherichia coli cells containing aspartase activity with kappa-carrageenan. Enzymic properties and application for L-aspartic acid production. Biochim Biophys Acta. 1979 Sep 12;570(1):179–186. [Abstract] [Google Scholar]
  • Tosa T, Sato T, Mori T, Yamamoto K, Takata I, Nishida Y, Chibata I. Immobilization of enzymes and microbial cells using carrageenan as matrix. Biotechnol Bioeng. 1979 Oct;21(10):1697–1709. [Abstract] [Google Scholar]
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