Basidiomycetous yeast Pseudozyma strains are often isolated from leaf surfaces. Here, we describe the sources of Pseudozyma yeasts and their useful secreted products, including enzymes and biosurfactants. We then outline the life of Pseudozyma on the leaf surface and introduce studies to verify ecological functions of their useful products. In addition, the function of Pseudozyma in maintaining the health of plants is briefly explained. Finally, the gene manipulation techniques necessary for future research and development of technological applications of Pseudozyma are described. Keywords: phylloplane; esterase; mannosylerythritol lipid; Pseudozyma; Pseudozyma antarctica