Table 1.
List of Pyrenochaeta spp. fungus sequences (ITS, SSU, and LSU) obtained from NCBI and Q-bank for phylogenetic trees.
Table 2.
Primers and gene size (bp) of antifungal resistance genes that used for PCR amplification.
Fig 1.
Colonial characteristic and microscopic morphology of P. unguis-hominis UM 256.
Colonial morphology (A) front and (B) reverse of P. unguis-hominis UM 256 on SDA after 14-day incubation. In microscopic view, UM 256 showed (C) pycnidia, (D) mature pycnidia with short septate setae (arrow), (E) mature pycnidia and conidia, and (F) pycnidia wall with textura angularis (630× magnification, bars 20 μm).
Fig 2.
Bayesian phylogenetic tree of Pyrenochaeta sp. based on the combined genes of ITS, SSU and LSU sequenced data.
The phylogenetic tree were constructed with 12 Pyrenochaeta species. The tree is rooted with C. hispidulum and S. terrestris as outgroup. The numbers on the nodes indicate Bayesian posterior probabilities.
Fig 3.
KOG and KEGG classifications of proteins in P. unguis-hominis UM 256.
(A) KOG class annotation distribution of P. unguis-hominis UM 256 genome. A: RNA processing and modification; B: Chromatin structure and dynamics; C: Energy production and conversion; D: Cell cycle control, cell division, chromosome partitioning; E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism; G: Carbohydrate transport and metabolism; H: Coenzyme transport and metabolism; I: Lipid transport and metabolism; J: Translation, ribosomal structure and biogenesis; K: Transcription; L: Replication, recombination and repair; M: Cell wall/membrane/envelope biogenesis; N: Cell motility; O: Post-translational modification, protein turnover, chaperones; P: Inorganic ion transport and metabolism; Q: Secondary metabolites biosynthesis, transport and catabolism; R: General function prediction only; S: Function unknown; T: Signal transduction mechanisms; U: Intracellular trafficking, secretion, and vesicular transport; V: Defense mechanisms; W: Extracellular structures; X: Unnamed protein and Z: Cytoskeleton. (B) Distribution of predicted proteins from P. unguis-hominis UM 256 genome that involved in metabolic pathway by KEGG database.
Table 3.
Transposable elements predicted in P. unguis-hominis UM 256.
Fig 4.
Phylogenomic analysis of P. unguis-hominis UM 256.
The phylogenomic tree was constructed with total of 23 fungi including four from Sordariomycetes, nine from Dothideomycetes, eight from Eurotiomycetes and two outgroups from the Saccharomycetes (C. albicans and S. cerevisiae).
Fig 5.
MAT1-2-1 gene of P. unguis-hominis UM256.
Genes constituting the MAT locus: MAT1-2-1 (UM256_7301), DNA lyase, Apn2 (UM 256_7302) and cytochrome C oxidase subunit Vla, Cox13 (UM256_7303).
Fig 6.
CAZymes class distribution of P. unguis-hominis and others Dothideomycetes.
(A) Distribution of each of the CAZymes family among P. unguis-hominis UM 256 and other Dohideomycetes fungi. (B) Distribution of CAZymes family involved in plant cell wall degradation. (C) Auxiliary group (AA) of CAZymes distribution among P. unguis-hominis UM 256 and others Dothideomycetes fungi. AA, auxiliary activities; CBM, carbohydrate-binding modules; CE, carbohydrate esterases; GH, glycoside hydrolases; GT, glycosyltransferases; PL, polysaccharide lyases. C, cellulose; H, Hemicellulose; P, pectin; HP, enzymes that degrade hemicellulose of pectin side chain.
Table 4.
Peptidase and genes that involved in keratin degradation of P. unguis-hominis UM 256.
Table 5.
Amino acid substitution detected in ERG 11/CYP51 genes of P. unguis-hominis UM 256.