Table 1.
Information on aquatic hyphomycete strains.
Table 2.
Information on A. pulchella standards, their Ct values and detection frequency.
Fig 1.
1a and 1b. Fluorescence profiles for the 6-carboxy-fluorescein fluorophore (FAM) as a function of PCR cycle and time. Each tested species and the negative controls are represented by a different color. A signal was classified as true positive if it was substantially higher than the initial fluorescence baseline [39], which was set automatically and individually for each sample by the qPCR device. Please note that the higher Ct value of A. pulchella in Fig 1b resulted from an extract with less template DNA.
Fig 2.
Standard curve for quantitative A. pulchella standards.
The standard curve (solid line) is displayed as mean Ct values (± SD; n = 7) of quantitative standards of A. pulchella (dilutions 4−1 to 4−8; filled circles) as a function of the calculated number of PCR forming units (PFU) in each PCR reaction (linear model r2 was 0.9994; p < 0.001). Non-quantitative calibrants (dilutions 4−9 to 4−12; open circles) that are not included in the standard curve are also visualized and connected by a dotted line. The limit of detection (LoD; 3 PFU; 9.9 fg DNA) and limit of quantification (LoQ; 47 PFU; 155.0 fg DNA) are indicated by arrows.