Fig 1.
Symptoms on sentinel nursery trees.
Leaf necrosis on Buxus microphylla (a); canker on Fraxinus chinensis (b); leaf necrosis on Ilex cornuta var. fortunei (c); and bark necrosis on Zelkova schneideriana (d).
Fig 2.
Heatmap of the abundance of fungal species identified by culturing analysis from symptomatic host tissues.
Darker colour indicate a higher abundance of the isolates. Zelkova schneideriana (ZS); Ilex cornuta var. fortunei (IC); Buxus microphylla (BM); Fraxinus chinensis (FC).
Fig 3.
Neighbour-joining trees of the OTU82 (from NGS analysis), the ITS sequence of isolate of Pseudonectria foliicola from this study, and top BLAST hits species (with GenBank accession numbers).
Numbers above branches represent bootstrap support for the nodes and posterior probability based on Bayesian analysis of the dataset (in bold). ¥ Sequence with 100% of identity with P. foliicola.
Fig 4.
Rarefaction curve of the OTUs from each of the four sampled species.
Table 1.
Taxonomic position, lifestyle, occurrence and recorded hosts/substrate for each of the eighteen fungal species identified by NGS analysis.
Fig 5.
Heatmap of the abundance of the fungal taxa identified as species by NGS analysis from sentinel nursery plants.
Higher intensities of the colour reveal higher abundances of the isolates. Zelkova schneideriana (ZS); Ilex cornuta var. fortunei (IC); Buxus microphylla (BM); Fraxinus chinensis (FC).* Ambiguous species identification, possible new species.