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Clonostachys rosea Strain ST1140: An Endophytic Plant-Growth-Promoting Fungus, and Its Potential Use in Seedbeds with Wheat-Grain Substrate

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Abstract

In this study, Clonostachys rosea strain ST1140, a naturally occurring endophyte in healthy roots of a pistachio tree, was identified morphologically and molecularly through DNA sequencing, and its endophytic ability and growth effect in/on three solanaceous plant species were tested. Three different organic substrates (bread wheat-grain, sawdust, and leonardite) were also investigated for their utility in cultivating and multiplying the strain. In-tray and pot experiments, the rates of seed germination and vegetative development of pepper, tomato, and eggplant inoculated with C. rosea at planting were compared to those of non-inoculated controls. In pot experiments, inoculating seedbeds with increasing doses of C. rosea strain ST1140 with wheat-grain substrate resulted in higher plant height values for all plant species, and the strain endophytically colonized the roots of all plant species. In-tray experiments revealed that ST1140 inoculation resulted in 2–4 days earlier and 10% higher germination rates for all plant species, as well as more vigorous and accelerated seedling growth (10–13 days earlier for seedlings at the 4–5 true leaf stage) on all inoculated plant species. Among three different organic substrates, wheat-grain was found to be the most effective for long-term cultivation and multiplication of the fungus, which could be of interest for its development as a commercial product. These results promised the strain ST1140’s use as a biofertilizer in seedbeds with a wheat-grain substrate.

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Authors and Affiliations

  1. GAP Agricultural Research Institute, 63040, Şanlıurfa, Turkey

    Şahimerdan Türkölmez

  2. Department of Plant Protection, Faculty of Agriculture, Bolu Abant Izzet Baysal University, 14030, Bolu, Turkey

    Göksel Özer

  3. Department of Plant and Animal Production, Vocational School of Kızıltepe, Mardin Artuklu University, 47000, Mardin, Turkey

    Sibel Derviş

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  1. Şahimerdan Türkölmez
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All authors contributed to the study’s conception and design, and approved the manuscript. ŞT performed experiments and collected data. GÖ performed molecular identification and data analyses and contributed to editing the manuscript. SD supervised the study and wrote the paper.

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Correspondence to Sibel Derviş.

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Türkölmez, Ş., Özer, G. & Derviş, S. Clonostachys rosea Strain ST1140: An Endophytic Plant-Growth-Promoting Fungus, and Its Potential Use in Seedbeds with Wheat-Grain Substrate. Curr Microbiol 80, 36 (2023). https://doi.org/10.1007/s00284-022-03146-3

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