Skip to main content
SpringerLink
Log in

Strain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate

  • Research
  • Published:
International Microbiology Aims and scope Submit manuscript

Abstract

Erythritol has been produced by various microorganisms including Yarrowia, Moniliella, Aureobasidium, and Candida strains. Due to its relatively high price, erythritol sweetener is used lesser than other polyols despite having many advantages. Therefore, in this study, Moniliella pollinis strain was improved for erythritol production by chemical mutagenesis and subsequently screening for cost-effective carbon sources for the enhanced erythritol yield. M. pollinis was subjected to N-methyl N-nitro N-nitroso guanidine (NTG), ethyl methyl sulfonate (EMS), and UV mutagenesis for improved erythritol production. The fmutant strains were evaluated for enhanced erythritol production medium optimization by using different carbon substrates at the shake flask level. To enhance the production of erythritol and statistical media, optimization was carried out using a central composite design (CCD). Among 198 isolated mutants, Mutant-58 strain generated by EMS mutagenesis was selected for further assessment. The Mutant-58 strain showed significant morphological changes as compared to the parent strain. Furthermore, statistically optimized media composition resulted in the higher production of erythritol (91.2 ± 3.4 g/L) with a yield of 40.7 ± 3.4 % in shake flask experiments. The optimized medium composition for erythritol production constitutes (g/L) 225 jaggery, 4.4 yeast extract (YE), 4.4 KH2PO4, 0.31 MgSO4, and pH 5.5. The present study demonstrated strain improvement, media, and process optimization resulting in a 30% increase in the erythritol production in the Mutant-58 as compared to the parent strain. This is also the first instance where jaggery has been used as a cost-effective carbon source alternative to glucose for industrial-scale erythritol production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abdelgalil SA, Attia AM, Reyed RM, Soliman NA, El Enashasy AH (2018) Application of experimental designs for optimization the production of Alcaligenes faecalis nyso laccase. J Sci Ind Res 77:713–722

    CAS  Google Scholar 

  • Bernt WO, Borzelleca JF, Flamm G, Munro IC (1996) Erythritol, a review of biological and toxicological studies. Regul Toxicol Pharmacol 24:S191–S197

    Article  CAS  PubMed  Google Scholar 

  • Burschäpers J, Schustolla D, Schügerl K, Röper H, De Troostembergh JC (2002) Engineering aspects of the production of sugar alcohols with the osmophilic yeast Moniliella tomentosa var pollinis, Part 2 batch and fed-batch operation in bubble column and airlift tower loop if reactors. Process Biochem 38:559–570

    Article  Google Scholar 

  • Bustos G, Moldes AB, Alonso JL, Vázquez M (2004) Optimization of D-lactic acid production by Lactobacillus coryniformis using response surface methodology. Food Microbiol 21:143–148

    Article  CAS  Google Scholar 

  • Choudhari S, Singhal R (2008) Medium optimization for the production of β-carotene by Blakeslea trispora, a statistical approach. Bioresour Technol 99:722–730

    Article  CAS  PubMed  Google Scholar 

  • Deshpande MS, Kulkarni PP, Kumbhar PS, Ghosalkar AR (2022) Erythritol production from sugar based feedstocks by Moniliella pollinis using lysate of recycled cells as nutrients source. Process Biochem 112:45–52

    Article  CAS  Google Scholar 

  • Domingues L, Vicente AA, Lima N, Teixeira JA (2000) Applications of yeast flocculation in biotechnological processes. Biotechnol Bioprocess Eng 5:288–305

    Article  CAS  Google Scholar 

  • Ghezelbash GR, Nahvi I, Emamzadeh R (2014a) Improvement of erythrose reductase activity, deletion of by-products and statistical medium optimization for enhanced erythritol production from Yarrowia lipolytica mutant. Curr Microbiol 49:149–157

    Article  Google Scholar 

  • Ghezelbash GR, Nahvi I, Malekpour A (2014b) Erythritol production with minimum By-product using Candida magnoliae mutant. Appl Biochem Microbiol 50:292–296

    Article  CAS  Google Scholar 

  • Hirata Y, Igarashi K, Ezaki S, Atomi H, Imanaka T (1999) High-level production of erythritol by strain 618A-01 isolated from pollen. J Biosci Bioeng 87:630–635

    Article  CAS  PubMed  Google Scholar 

  • Hirpara P, Thakare N, Patel D, Kele VD (2020) Jaggery, a natural sweetener. J Pharmacogn Phytochem. 9:3145–3148

    CAS  Google Scholar 

  • Ishizuka H, Wako K, Kasumi T, Sasaki T (1989) Breeding of a mutant of Aureobasidium sp with high erythritol production. J Ferment Bioeng 68:310–314

    Article  CAS  Google Scholar 

  • Jagannadha Rao PVK, Das M, Das SK (2007) Jaggery-a traditional Indian sweetner. Ind J Trad Knowl 6:95–102

    Google Scholar 

  • Kennard M, Janekeh M (1991) Two- and three-phase mixing in a concentric draft tube gas-lift fermentor. Biotechnol Bioeng 38:261–1270

    Article  Google Scholar 

  • Kobayashi O, Yoshimoto H, Sone H (1999) Analysis of the genes activated by the FLO8 gene in Saccharomyces cerevisiae. Curr Genet 36:256–261

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi Y, Iwata H, Mizushima D, Ogihara J, Kasumi T (2015) Erythritol production by Moniliella megachiliensis using nonrefined glycerol waste as carbon source. Lett Appl Microbiol 60:475–480

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi Y, Yoshida J, Iwata H, Koyama Y, Kato J, Ogihara J, Kasumi T (2013) Gene expression and function involved in polyol biosynthesis of Trichosporonoides megachiliensis under hyper-osmotic stress. J Biosci Bioeng 115:645–650

    Article  CAS  PubMed  Google Scholar 

  • Krajangsod S, Chotikamas S, Tawai A, Sriariyanun M (2018) Measurement and thermodynamic modelling of erythritol solubility in aqueous solvents. Orient J Chem 34:265–275

    Article  CAS  Google Scholar 

  • Lee K, Kim Y, Ryu W, Seo H, Kim H (2003) Purification and characterization of a novel erythrose reductase from Candida magnoliae. Appl Environ Microbiol 69:3710–3718

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lin SJ, Wen CY, Wang PM, Huang JC, Wei CL, Chang JW, Chu WS (2010) High-level production of erythritol by mutants of osmophilic Moniliella sp. Process Biochem 45:973–979

    Article  CAS  Google Scholar 

  • Livesey G (2003) Health potential of polyols as sugar replacers, with emphasis on low glycaemic properties. Nutr Res Rev 16:163–191

    Article  CAS  PubMed  Google Scholar 

  • Mirończuk AM, Dobrowolski A, Rakicka M, Rywińska A, Rymowicz W (2015a) Newly isolated mutant of Yarrowia lipolytica MK1 as a proper host for efficient erythritol biosynthesis from glycerol. Process Biochem 50:61–68

    Article  Google Scholar 

  • Mirończuk AM, Rakicka M, Biegalska A, Rymowicz W, Dobrowolski A (2015b) A two-stage fermentation process of erythritol production by yeast Y lipolytica from molasses and glycerol. Bioresour Technol 198:445–455

    Article  PubMed  Google Scholar 

  • Nath A, Dutta D, Kumar P, Singh JP (2015) Review on recent advances in value addition of jaggery based products. J Food Proc and Technol 6:1–2

    Google Scholar 

  • Ookura T, Azuma K, Isshiki K, Taniguchi H, Kasumi T, Kawamura Y (2005) Primary structure analysis and functional expression of erythrose reductases from erythritol-producing fungi (Trichosporonoides megachiliensis SNG-42). Biosci Biotechnol Biochem 69:944–951

    Article  CAS  PubMed  Google Scholar 

  • Otey FH, Sloan JW, Wilham CA, Mehltretter CL (1961) Erythritol and ethylene glycol from dialdehyde starch. Ind Eng Chem 5:267–268

    Article  Google Scholar 

  • Rakicka-Pustułka M, Mirończuk A, Celińska E, Białas W, Rymowicz W (2020) Scale-up of the erythritol production technology – process simulation and techno-economic analysis. J Clean Prod 257:1–11

    Article  Google Scholar 

  • Restiawaty E, Gani KP, Dewi A, Arina LA, Kurniawati KI, Budhi YW, Akhmaloka (2020) Bioethanol production from sugarcane bagasse using Neurospora intermedium in an airlift bioreactor. Int J Renew Energy Dev 9:247–253

    Article  CAS  Google Scholar 

  • Russell I, Stewart GG, Reader HP, Johnston JR, Martin PA (1980) Revised nomenclature of genes that control yeast flocculation. J Inst Brew 86:120–121

    Article  Google Scholar 

  • Rzechonek DA, Dobrowolski A, Rymowicz W, Mirończuk AM (2017) Recent advances in biological production of erythritol. Crit Rev Biotechnol 38:620–633

    Article  PubMed  Google Scholar 

  • Sandra RP, Gabriela SS, Jazmina CRA, Helen FL, Rita CRG (2017) Production of melanin pigment by fungi and its biotechnological applications, 1st edn. InTech, Melanin, London, UK, pp 47–75

    Google Scholar 

  • Savergave LS, Gadre RV, Vaidya BK, Narayanan K (2011) Strain improvement and statistical medium optimization for enhanced erythritol production with minimal by-products from Candida magnoliae mutant R23. Biochem Eng J 55:92–100

    Article  CAS  Google Scholar 

  • Shindou T, Sasaki Y, Miki H, Eguchi T, Hagiwara K, Ichikawa T (1988) Determination of erythritol in fermented foods by high performance liquid chromatography. Food Hyg Saf Sci (Shokuhin Eiseigaku Zasshi) 29:419

    Article  CAS  Google Scholar 

  • Shukla SA (2015) Bradford multiplexing method for protein estimation in fermented foods, Soy sauces. Bangladesh J Pharmacol 11:24–27

    Article  Google Scholar 

  • Singh J (2013) Manufacturing jaggery, a product of sugarcane, as health food. Agrotechnology 1:1–3

    Article  Google Scholar 

  • Sivaraman B, Badle S, Rangaswamy V (2016) Production of 1,3-butadiene from erythritol. Chemistry Select 1:4630–4632

    CAS  Google Scholar 

  • Thesseling FA, Bircham PW, Mertens S, Voordeckers K, Verstrepen KJ (2019) A hands-on guide to brewing and analyzing beer in the laboratory. Curr Prot Microbiol 54:91

    Article  Google Scholar 

  • Trenner NR BF (1951) Hydrogenation of tartaricacid esters to erythritol. 1951, US2571967A.

  • Vagenende V, Yap MGS, Trout BL (2009) Mechanisms of protein stabilization and prevention of protein aggregation by glycerol. Biochemistry 48:11084–11096

    Article  CAS  PubMed  Google Scholar 

  • Verstrepen KJ, Derdelinckx G, Verachtert H, Delvaux FR (2003) Yeast flocculation, what brewers should know. Appl Microbiol Biotechnol 61:197–205

    Article  CAS  PubMed  Google Scholar 

  • Vicente AA, Dluhý M, Teixeira JA (1999) Increase of ethanol productivity in an airlift reactor with a modified draught tube. Can J Chem Eng 77:497–502

    Article  CAS  Google Scholar 

  • Vijayendra SVN, Bansal D, Prasad MS, Nand K (2001) Jaggery, a novel substrate for pullulan production by Aureobasidium pullulans. CFR-77. Process Biochem 37:359–364

    Article  CAS  Google Scholar 

  • Vohra A, Satyanarayana T (2002) Statistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala. Process Biochem 37:999–1004

    Article  CAS  Google Scholar 

  • Winston F (2008) EMS and UV mutagenesis in yeast. In, Curr Protoc Mol Biol Wiley, New York City, US, pp 133B1–133B5

    Google Scholar 

  • Yang SW, Park JB, Soo Han N (1999) Production of erythritol from glucose by an osmophilic mutant of Candida magnoliae. Biotechnol Lett 21:887–890

    Article  CAS  Google Scholar 

  • Yoshida H, Sugahara THJ (1984) Studies on free sugars and free sugar alcohols of mushrooms. Nippon Shokuhin Koyko Gakkaishi 31:765–771

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the Directors of CSIR-NCL, Pune., Academy of Scientific and Innovative Research (AcSIR), Ghaziabad- 201002, India, and High Value Chemicals group, Reliance Industries Limited, Ghansoli, Navi Mumbai, India, 400701 for infrastructure and support.

Author information

Authors and Affiliations

  1. NCIM-Resource Center, Biochemical Sciences Division, CSIR-National Chemical Laboratory, -411008, Pune, India

    Anil B. Khatape & Syed G. Dastager

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Anil B. Khatape & Syed G. Dastager

  3. High Value Chemicals group, Reliance Industries Limited, Ghansoli, Navi Mumbai, 400701, India

    Anil B. Khatape & Vidhya Rangaswamy

Authors
  1. Anil B. Khatape
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vidhya Rangaswamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Syed G. Dastager
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Anil Khatape designed, carried out the experiments, and wrote the original draft. Vidhya Rangaswamy visualized and supervised the experiments and reviewed and edited the manuscript. Syed Dastager visualized and supervised the experiments and reviewed and edited the manuscript. All authors have approved the final version of the manuscript.

Corresponding author

Correspondence to Syed G. Dastager.

Ethics declarations

Ethical approval

This article does not contain any studies with human participants or vertebrates.

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khatape, A.B., Rangaswamy, V. & Dastager, S.G. Strain improvement for enhanced erythritol production by Moniliella pollinis Mutant-58 using jaggery as a cost-effective substrate. Int Microbiol 27, 581–596 (2024). https://doi.org/10.1007/s10123-023-00411-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10123-023-00411-8

Keywords

Search

Navigation