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Species diversity of Colletotrichum infecting Annona spp. in Brazil

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Abstract

Anthracnose disease caused by Colletotrichum species is a limiting factor in the production and commercialization of annonaceous crops in different regions of the world. In this study, 96 Colletotrichum isolates were collected from soursop and sugar apple leaves showing anthracnose symptoms in commercial plantations of the state of Alagoas, northeastern region of Brazil. To assess initially the species diversity, the isolates were identified by phylogeny based on the sequences of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. Representative strains were selected for further identification based on multigene phylogeny analysis using sequences of the internal transcribed spacer region (ITS), actin (ACT), chitin synthase (CHS-1), β-tubulin (TUB2), calmodulin (CAL), histone3 (HIS3), glutamine synthetase (GS) and (GAPDH) genes. Morpho-cultural characterization and pathogenicity tests were also performed. These analyses allowed the identification of nine species: Colletotrichum brevisporum, C. cliviae, C. fructicola, C. gigasporum, C. gloeosporioides, C. karstii, C. siamense, C. theobromicola and C. tropicale. Until now, only C. fructicola, C. gloeosporioides and C. theobromicola had been associated with anthracnose of soursop and sugar apple in Brazil. C. brevisporum, C. cliviae and C. gigasporum species are also described for the first time on annonaceous species worldwide.

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  • 07 August 2019

    Due to an unforeseen oversight, the articles listed below were initially incorrectly published in Vol. 153: Issue 1, This has been corrected.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de Alagoas - FAPEAL. We also thank the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES for a doctoral fellowship.

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  1. Centro de Ciências Agrárias, Universidade Federal de Alagoas, Br 104, km 85 norte, Rio Largo, AL, 57100-000, Brazil

    Jaqueline F. O. Costa, Sandra H. Kamei, Janaíne Rossane A. Silva, Ana Raphaela G. da S. Miranda, Mariote B. Netto, Sarah Jacqueline C. da Silva, Gaus S. de Andrade Lima & Iraildes P. Assunção

  2. Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Campus Crato, Rua Vereador Sebastião Maciel Lopes, s/n - Bairro São José, Crato, CE, 63133-610, Brazil

    Kamila C. Correia

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  1. Jaqueline F. O. Costa
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Costa, J.F.O., Kamei, S.H., Silva, J.R.A. et al. Species diversity of Colletotrichum infecting Annona spp. in Brazil. Eur J Plant Pathol 153, 1119–1130 (2019). https://doi.org/10.1007/s10658-018-01630-w

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