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Lasiodiplodia spp. associated with Aquilaria crassna in Laos

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Abstract

Aquilaria (Thymelaeaceae) is a genus of evergreen trees, which is well-known in producing resinous heartwood (known as agarwood). Infection by Lasiodiplodia spp. has been reported effectively inducing the agarwood formation in members of Aquilaria. In this study, 12 taxa of Lasiodiplodia were retrieved from trunks of healthy, brown, and ligaloes tissue of Aquilaria crassna in Laos, of which eight species are new to science. Fungal identification was conducted using a combination of morphology and multi-locus phylogenetic analyses based on combined translation elongation factor 1-a sequence (tef1-a), internal transcribed spacer (ITS), ß-tubulin (TUB), and RNA polymerase II beta subunit (RPB2) nuDNA sequences. Cashew nut-shaped conidia and the baculate ornamentation on the conidia of Lasiodiplodia spp. are first reported in this study. Aquilaria crassna proved a good host for Lasiodiplodia, and the function of Lasiodiplodia spp. in inducing agarwood formation needs further study.

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Acknowledgements

Dr. Jinlong Zhang (KFBG) is acknowledged for host plant identification.

Funding

This work was supported by the Fundamental Research Funds for the Central Universities (No. 2016ZCQ04), National Natural Science Foundation of China (General Program, 31770015, 31370063), NSFC Projects of International Cooperation and Exchanges (3155461143028), and National Science and Technology Foundation Project (2014FY210400).

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Author notes

  1. Yu Wang and Sen Lin contributed equally to this work.

Authors and Affiliations

  1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Yu Wang & Yu-Cheng Dai

  2. Institute of Microbiology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Yu Wang & Ying Zhang

  3. Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Sen Lin, Lin Zhao & Wei He

  4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Xiang Sun

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  1. Yu Wang
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  4. Xiang Sun
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Correspondence to Ying Zhang or Yu-Cheng Dai.

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Wang, Y., Lin, S., Zhao, L. et al. Lasiodiplodia spp. associated with Aquilaria crassna in Laos. Mycol Progress 18, 683–701 (2019). https://doi.org/10.1007/s11557-019-01481-7

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  • DOI: https://doi.org/10.1007/s11557-019-01481-7

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