Abstract
Two Tolypocladium species, Tolypocladium reniformisporum sp. nov., and T. cylindrosporum, were isolated from the sclerotium of a naturally occurring Ophiocordyceps sinensis specimen in the Baima Snow Mountains of Deqin County, Yunnan Province in southwestern China. They were described and illustrated by morphological characters and growth-temperature relationships. Phylogenetic analyses based on a 5-gene (SSU, LSU, TEF, RPB1, and RPB2) dataset and sequences of the internal transcribed spacer regions (ITS) showed that T. reniformisporum was phylogenetically closely related to T. cylindrosporum and other similar species. Tolypocladium reniformisporum was morphologically distinguishable from other Tolypocladium species because of its slow-growing colonies, short phialides, and smaller reniform conidia. Growth tests showed that the isolated T. reniformisporum and T. cylindrosporum strains were psychrotrophic, with optimal growth temperatures of 18–22 ℃, and minimum growth temperatures below 5 ℃.
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References
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The authors received funding support from the National Natural Science Foundation of China (grant nr. 31760011), the Ministry of Science and Technology of the People’s Republic of China (nr. KC1610530), the China Postdoctoral Science Foundation (nr. 2017M613017), and the Research Innovation Fund for Graduate Students of Yunnan University (nr. YDY17100).
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The study conception and design was done by Yuan-Bing Wang and Hong Yu. Material preparation, data collection and analysis were performed by Yao Wang, De-Xiang Tang, Yuan-Bing Wang and Yan-Fang Liu. The first draft of the manuscript was written by Yan-Fang Liu and Yao Wang, and the revision and perfection of the manuscript were performed by Yan-Fang Liu and Yao Wang. Alison E. M. Adams provided some good suggestions and polished the language of the manuscript. All authors commented on previous versions of the manuscript and all authors read and approved the final manuscript.
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Section editor: Hans-Josef Schroers
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Wang, Y., Liu, YF., Tang, DX. et al. Tolypocladium reniformisporum sp. nov. and Tolypocladium cylindrosporum (Ophiocordycipitaceae, Hypocreales) co-occurring on Ophiocordyceps sinensis. Mycol Progress 21, 199–214 (2022). https://doi.org/10.1007/s11557-021-01675-y
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DOI: https://doi.org/10.1007/s11557-021-01675-y