Abstract
Endophytes of healthy Picea needles were isolated in a mycological survey within the Acadian forest of southeastern New Brunswick, Canada. The goal of the survey was to better characterize conifer endophyte diversity and their natural products. Many new species and natural products have been discovered while illuminating that the life histories of these poorly studied fungi are more complex than expected. From the hitherto undescribed species Niesslia endophytica collected from P. mariana, chlorinated and non-chlorinated resorcylic acid lactones were detected by HRMS and radicicol (1), radicicols B (2), and C (3) were characterized. Strasseria geniculata isolated from P. rubens produced the polyketides aspergillumarins A (4) and B (5), the first report of these secondary metabolites from the fungus. Radicicol (1) and aspergillumarins A/B (2,3) had moderate antifungal activity, and no purified compounds had phytotoxic activity. Endophytes of conifers continue to be a reservoir for new fungi and fascinating chemical ecologies.
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The datasets generated during and/or analyzed during the current study are available from the authors on request.
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Acknowledgements
We thank Grace Daly and Djay Chevannes (Carleton University) for technical assistance with the isolation of natural products and Annie Dicaire (Pacific Forestry Centre) for assisting in DNA extraction and PCR. We also thank Drs. Justin Renaud and Mark Sumarah (Agriculture and Agri-Food Canada, London) for the acquisition of HRMS data. We thank Dr. Keith A. Seifert for supporting this work, Rafik Assabgui for accessioning cultures into the DAOMC collection, and the MMTL group of ORDC-AAFC, Ottawa, for processing DNA sequencing.
Funding
This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC SYN 479724-15, NSERC CRDPJ 421782-11) and MITACS (IT06326).
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J. David Miller, Joey Tanney, and David McMullin contributed to the study conception and design. Data collection and analysis were performed by Joey Tanney, David McMullin, and Jordan DiStefano. The first draft of the manuscript was written by Joey Tanney and David McMullin. All authors read and approved the final manuscript.
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Tanney, J.B., Di Stefano, J., Miller, J.D. et al. Natural products from the Picea foliar endophytes Niesslia endophytica sp. nov. and Strasseria geniculata. Mycol Progress 22, 17 (2023). https://doi.org/10.1007/s11557-023-01869-6
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DOI: https://doi.org/10.1007/s11557-023-01869-6