Abstract
Freshwater fungus Anguillospora longissima was recovered as root endophytic fungus from the pteridophytic plant Equisetum sp., growing in riparian areas of Nainital, Kumaun Himalaya, and evaluated through well diffusion and dual culture methods. The isolated endophytic fungus was found a potent inhibitor for test pathogenic fungi. Maximum inhibition by the isolated endophytic fungus was showed against pathogenic fungus Tilletia indica (79.76%), while minimum was recorded against Fusarium oxysporum (35.73%), in dual culture method. Further, in well diffusion method, pathogenic fungi Colletotrichum falcatum and Pyricularia oryzae were found maximum (62.96%) and minimum (17.85%) inhibited fungi, respectively, by the isolated fungus. In the present investigation, the used root endophytic fungus A. longissima was found potent to produce some antifungal substances that inhibited the growth of all test pathogenic fungi.
References
Bull AT, Goodfellow M, Slater JH (1992) Biodiversity as a source of innovation in biotechnology. Annu Rev Microbiol 46:219–252
Aly AH, Debbab A, Proksch P (2011) Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol 90(6):1829–1845
Demain AL (2000) Microbial natural products: a past with a future. In: Wrigley SK, Hayes MA, Thomas R, Chrystal EJT, Nicholson N (eds) Biodiversity: new leads for pharmaceutical and agrochemical industries. The Royal Society of Chemistry, Cambridge, pp 3–16
Calvo AM, Wilson RA, Bok JW, Keller NP (2002) Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev 66:447–459
Hoffmeister D, Keller NP (2007) Natural products of filamentous fungi: enzymes, genes and their regulation. Nat Prod Rep 24:393–416
Liu X, Dong M, Chen X, Jiang M, Zhou J (2008) Antimicrobial activity of an endophytic Xylaria sp. YX-28 and identification of its antimicrobial compound 7-amino-4-methylcoumarin. App Microbiol Biotechnol 78:241–247
Strobel GA, Daisy B (2003) Bio-prospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67:491–502
Li Y, Song YC, Liu JY, Ma YM, Tan RX (2005) Anti Helicobacter pylori substances from endophytic fungal cultures. World J Microbiol Biotechnol 21:553–558
Vaz ABM, Mota RC, Bomfim MRQ (2009) Antimicrobial activity of endophytic fungi associated with Orchidaceae in Brazil. Can J Microbiol 55(12):1381–1391
Dreyfuss MM, Chapela IH (1992) Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals. Biotechnol Ser 26:49–80
Zou WX, Meng JC, Lu H, Chen GX, Shi GX, Zhang TY, Tan RX (2000) Metabolites of Colletotrichum gloeosporioides, an endophytic fungus in Artemisia mongolica. J Nat Prod 63:1529–1530
Bugni TS, Ireland CM (2004) Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Nat Prod Rep 21:143–163
Chowdhary K, Kaushik N, Coloma AG, Raimundo CM (2012) Endophytic fungi and their metabolites isolated from Indian medicinal plant. Phytochem Rev 11:101–112
Ingold CT (1942) Aquatic hyphomycetes of decaying alder leaves. Trans Br Mycol Soc 25:339–417
Webster J, Descals E (1981) Morphology, distribution and ecology of conidial fungi in freshwater habitats. In: Cole GT, Kendrick B (eds) The biology of conidial fungi. Academic Press, New York, pp 295–355
Maranova L, Barlocher F (1988) Hyphomycetes from Canadian streams. I. Basidiomycetous Anamorphs. Mycotaxon 32:339–351
Ingold CT (1975) An illustrated guide to aquatic and water borne hyphomycetes (fungi imperfecti) with notes on their biology. Freshwater Biological Association Scientific Publication No. 30, England, p 96
Waid JS (1954) Occurrence of aquatic hyphomycetes upon the roots surface of beech grown in woodland soil. Trans Br Mycol Soc 37:420–421
Sridhar KR, Barlocher F (1992) Endophytic aquatic hyphomycetes of roots of spruce, birch and maple. Mycol Res 96:305–308
Petrini O, Sieber TN, Toti L, Viret O (1992) Ecology, metabolite production, and substrate utilization in endophytic fungi. Nat Toxins 1:185–196
Sati SC, Pargaien N, Belwal M (2006) Three species of aquatic hyphomycetes as new root endophytes of temperate forest plants. Natl Acad Sci Lett 29:351–354
Harrigan GG, Armentrout BL, Gloer JB, Shearer CA (1995) Anguillosporal, a new antibacterial and antifungal metabolite from the freshwater fungus Anguillospora longissima. J Nat Prod 58:1467–1469
Fisher PJ, Anson AE (1983) Antifungal effects of Massarina aquatica growing on oak wood. Trans Br Mycol Soc 81:523–527
Shearer CA, Zare-Maivan H (1988) In vitro hyphal interactions among wood- and leaf-inhabiting ascomycetes and fungi imperfecti from freshwater habitats. Mycologia 80:31–37
Platas G, Pelaez F, Collado J, Villuendas G, Diez MT (1998) Screening of antimicrobial activities by aquatic hyphomycetes cultivated on various nutrient sources. Cryptogam Mycol 19:33–43
Gulis VI, Stephanovich AI (1999) Antibiotic effects of some aquatic hyphomycetes. Mycol Res 103:111–115
Singh L, Sati SC (2014) Bio-prospecting of root endophytic aquatic fungus Cylindrocarpon aquaticum as antibacterial potential. J Pure Appl Microbiol 8:4903–4908
Pandey A, Simon LS, Ahmad S (2009) Screening of antifungal activities in Trichoderma isolates against some soil borne plant pathogens. Nat Acad Sci India 33(9&10):285–288
Eleyinmi AF (2007) Chemical composition and antibacterial activity of Gongronema latifolium. J Zhejiang Univ Sci B 8(5):352–358
Sati SC, Arya P (2010) Antagonism of some aquatic hyphomycetes against plant pathogenic fungi. Sci World J 10:760–765
Tan RX, Zou WX (2001) Endophytes: a rich source of functional metabolites. Nat Prod Rep 18:448–459
Kumar S, Kaushik N, Edrada-Ebel R, Ebel R, Proksch P (2008) Endophytic fungi for pest and disease management. In: Ciancio A, Mukerji KG (eds) Integrated management of diseases caused by fungi phytoplasma and bacteria. Springer, Berlin, pp 365–387
Khan MA (1987) Interspecies interactions in aquatic hyphomycetes. Bot Mag Tokyo 100:295–303
Sati SC, Singh L (2014) Bioactivity of root endophytic freshwater hyphomycetes Anguillospora longissima (Sacc. and Syd.) Ingold. Sci World J 14:1–5
Zhang Y, Mu J, Feng Y, Kang Y, Zhang J, Gu P, Wang Y, Ma L, Zhu Y (2009) Broad-spectrum antimicrobial epiphytic and endophytic fungi from marine organisms: isolation, bioassay and taxonomy. Mar Drugs 7:97–112
Acknowledgements
Authors are thankful to University Grant Commission (UGC), New Delhi, for providing fellowship to LS under Meritorious Student Scheme. We are also thankful to Head of the Department, for providing necessary facilities during the work.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Singh, L., Sati, S.C. Antagonistic Activity of Isolated Root Endophytic Freshwater Fungus Anguillospora Longissima Against Pathogenic Fungi. Natl. Acad. Sci. Lett. 43, 201–205 (2020). https://doi.org/10.1007/s40009-019-00818-w
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40009-019-00818-w