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Thielaviopsis musarum causes postharvest crown and fruit rot of banana in Northeastern Brazil

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Abstract

Crown rot is an important postharvest disease of banana fruit caused by a fungal complex. The development of these microorganisms in the banana favors rot of the fruit and crown, affecting fruit quality and rendering the fruit unsafe to eat. Fungi that cause postharvest rot include species of the complex Thielaviopsis paradoxa. In March 2015, symptoms of crown rot were observed in banana grown in the state of Piauí, Northeastern Brazil. The fruits exhibited rot, usually with signs of a dark gray mycelium; fruits at an advanced stage of rot broke from the stem. Microscopic analysis revealed the presence of primary and secondary conidia as well as aleurioconidia, typical for Thielaviopsis spp.. The sexual phase was not observed. The ITS and TEF1-α genes were sequenced and subjected to phylogenetic analysis; consequently, the isolates were identified as Thielaviopsis musarum. In inoculated fruits, the fungus caused black rot, followed by a browning and softening of the pulp. This is the first record of postharvest rot in banana caused by T. musarum in Brazil.

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References

  • Chapola RG, Ogasawara GA, Jans B, Massola Junior NS (2014) Controle da podridão abacaxi da cana-de-açúcar por meio da pulverização de fungicidas em rebolos no sulco de plantio. Cienc Rural 44:197–202

    Article  Google Scholar 

  • Cordeiro ZJM, Matos AP (2005) Doenças da banana. Informe Agropecuário 26:12–16

    Google Scholar 

  • de Beer ZW, Duong TA, Barnes IBD, Wingfield BD, Wingfield MJ (2014) Redefining Ceratocystis and allied genera. Stud Mycol 79:187–219

    Article  PubMed  PubMed Central  Google Scholar 

  • Farr DF, Rosmman AY (2015) Fungal database, systematic mycology and microbiology LABORATORY, ARS, USDA. Available at: http://nt.ars-grin.gov/fungaldatabases/. Accessed on October 30, 2015

  • Greene GL, Goos RD (1963) Fungi associated with crown rot boxed bananas. Phytopathology 53:271–275

    Google Scholar 

  • Griffee PJ, Burden OJ (1976) Fungi associated with crown rot of boxed bananas in the Windward Islands. J Phytopathol 85:149–158

    Article  Google Scholar 

  • Hepperle D (2004) SeqAssem©. Win32-Version. a sequence analysis tool contig assembler and trace data visualization tool for molecular sequences. Available at: http://www.sequentix.de

  • Hirata T, Kimishima E, Aoki T, Nirenberg HI, O’Donnell K (2001) Morphological and molecular characterization of Fusarium verticillioides from rotten banana imported into Japan. Mycoscience 42:155–166

    Article  Google Scholar 

  • Jacobs K, Bergdahl DR, Wingfield MJ, Halik S, Seifert KA, Bright DE, Wingfield BD (2004) Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetles. Mycol Res 108:411–418

    Article  PubMed  Google Scholar 

  • Jimenez M, Logrieco A, Bottalico A (1993) Occurrence and pathogenicity of Fusarium species in banana fruits. J Phytopathol 137:214–220

    Article  Google Scholar 

  • Lassois L, Jijakli MH, Chillet M, Bellaire LL (2010) Crown rot of bananas: pre-harvest factors involved in postharvest disease development and integrated control methods. Plant Dis 94:648–658

    Article  Google Scholar 

  • Marin DH, Sutton TB, Blankenship SM, Swallow WH (1996) Pathogenicity of fungi associated with crown rot of bananas in Latin America on Grande Naine and disease-resistant hybrid bananas. Plant Dis 80:525–528

    Article  Google Scholar 

  • Matos AP (2000) Abacaxi produção: aspectos técnicos. Embrapa Mandioca e Fruticultura/Brasília: Embrapa Comunicação para Transferência de Tecnologia. 17–51

  • Mbenoum M, de Beer ZB, Wingfield MJ, Wingfield BD, Roux J (2014) Reconsidering species boundaries in the Ceratocystis paradoxa complex, including a new species from oil palm and cacao in Cameroon. Mycologia 106:757–784

    Article  Google Scholar 

  • McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W, Hawksworth DL, Herendeen PS, Knapp S, Marhold K, Prado J, van Prud’homme Reine WF, Smith GF, Wiersema JH, Turland NJ (2012) Regnum vegatabile international. code of nomenclature for algae, fungi, and plants (Melbourne code): 142. Koeltz Scientific Books, Germany

    Google Scholar 

  • Mendes MAS, da Silva VL, Dianese JC, Ferreira MASV, Santos CEN, Gomes Neto E, Urben AF, Castro C (1998) Fungos em Plantas no Brasil. Embrapa-SPI, Embrapa Cenargem, Brasília

    Google Scholar 

  • Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. Proc Gateway Comput Environ Workshop (GCE), New Orleans, LA 1–8

  • Mitchell RS (1937) Stem end rot of bananas with special reference to the physiological relationships of Thielaviopsis paradoxa (de Seynes) Von Hohn. J Council Sci Industr Res 10:123–130

    Google Scholar 

  • Moretti A, Mulè G, Susca A, Gonzalez-Jaén MT, Logrieco A (2004) Toxin profile, fertility and AFLP analysis of Fusarium verticillioides from banana fruits. Europ J Plant Pathol 110:601–609

    Article  CAS  Google Scholar 

  • Pinho DB, Dutra DC, Pereira OL (2013) Notes on Ceratocystis paradoxa causing internal post-harvest rot disease on immature coconut in Brazil. Trop Plant Pathol 38:152–157

    Article  Google Scholar 

  • Ploetz RC, Zentmayer GA, Nishijima WT, Rohrbach KG, Ohr HD (1994) Compendium of tropical fruit disease. APS Press. The American Phytopathological Society, St. Paul

    Google Scholar 

  • Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Syst Biol 53:793–808

    Article  PubMed  Google Scholar 

  • Rambaut A (2009) Fig Tree 1.2.2. Available at: http://tree.bio.ed.ac.uk/software/figtree/. Accessed on October 20, 2015

  • Riedl H (1962) Ceratocystis musarum sp. n., die heuptfruchtform der Thielaviopsis–Art von bananen stielen. Sydowia 15:247–251

    Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres D, Darling A, H¨ohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2011) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  Google Scholar 

  • Sambrook J, Fritschi EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Silva JR, Cordeiro ZJM (2000) Fitossanidade na exportação de banana. In: Borges AL, Mesquita ALM, Matos AP, Costa DC, Silva JR, Carvalho JEB, Gasparotto L, Fancelli M, Meissner Filho PE, Brioso PST, Cordeiro ZJM (eds) Banana Fitossanidade. Empresa Brasileira de Pesquisa Agropecuária. Embrapa Comunicação para Transferência de Tecnologia, Brasília, pp 9–14

    Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Upadhyay HP (1981) A monograph of Ceratocystis and Ceratocystiopsis. University of Georgia Press, Athens

    Google Scholar 

  • Van Hove F, Waalwijk C, Logrieco A, Munaut F, Moretti A (2011) Fusarium musae (Fusarium musae) sp. nov. a recently discovered species from banana is sister to F. verticillioides. Mycologia 103:570–585

    Article  PubMed  Google Scholar 

  • Warwick DRN, Passos EEM (2009) Outbreak of stem bleeding in coconuts caused by Thielaviopsis paradoxa in Sergipe, Brazil. Trop Plant Pathol 34:175–177

    Article  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academid Press, San Diego, pp 315–322

    Google Scholar 

Download references

Acknowledgments

The author thanks the Brazilian Federal Agency for the Support and Evaluation of Graduate Education (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES) for granting a PNPD fellowship and the Minas Gerais Research Foundation (Fundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIG) for financial support. The Culture Collection of Phytopathogenic Fungi at the Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco), for giving use isolated in this study.

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Authors and Affiliations

  1. Departamento de Fitotecnia, Universidade Federal do Piauí, Centro de Ciências Agrárias, Ininga, 64049-550, Teresina, PI, Brazil

    Maruzanete Pereira de Melo, José Evando Aguiar Beserra Júnior & Francisco de Alcântara Neto

  2. Instituto Nacional de Pesquisas da Amazônia-INPA, 69060-001, Manaus, AM, Brazil

    Kedma da Silva Matos

  3. Departamento de Fitopatologia, Universidade Federal de Lavras, 37200-000, Lavras, MG, Brazil

    Silvino Intra Moreira, Fabiano França Silva & Eduardo Alves

  4. Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-000, Viçosa, MG, Brazil

    Olinto Liparini Pereira

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  1. Maruzanete Pereira de Melo
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  2. Kedma da Silva Matos
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  3. José Evando Aguiar Beserra Júnior
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  4. Silvino Intra Moreira
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  5. Fabiano França Silva
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  6. Francisco de Alcântara Neto
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  7. Eduardo Alves
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Correspondence to Silvino Intra Moreira.

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Section Editor: Meike Piepenbring

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de Melo, M.P., da Silva Matos, K., Júnior, J.E.A.B. et al. Thielaviopsis musarum causes postharvest crown and fruit rot of banana in Northeastern Brazil. Trop. plant pathol. 41, 258–263 (2016). https://doi.org/10.1007/s40858-016-0094-4

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  • DOI: https://doi.org/10.1007/s40858-016-0094-4

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