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Xenodidymella iranica sp. nov. and new hosts of X. glycyrrhizicola in Iran

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Abstract

The genus Xenodidymella includes eight species that are mainly plant pathogens causing different diseases worldwide. During a survey on plant diseases in Khuzestan province in southwestern Iran, seven isolates of Xenodidymella were obtained from symptomatic plants, including Brassica oleracea var. capitata, Callistemon citrinus, Corchorus olitorius, Coriandrum sativum, Glycyrrhiza lepidota, and Ziziphus mauritiana. A multi-locus phylogenetic analysis based on the internal transcribed spacer regions 1 and 2 including the 5.8S nuclear ribosomal DNA (ITS), partial β-tubulin (tub2), and RNA polymerase II second largest subunit (rpb2), combined with morphology, was used to identify the isolates. Accordingly, the novel species Xenodidymella iranica is introduced and characterized here along with five new hosts for X. glycyrrhizicola. In pathogenicity tests, X. iranica and three strains of X. glycyrrhizicola are known as pathogenic on host plants from where they were originally isolated.

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Data availability

The data that support the findings of this study are openly available in GenBank at https://www.ncbi.nlm.nih.gov, MycoBank at https://www.mycobank.org/, and TreeBase at http://purl.org/phylo/treebase/phylows/study/TB2:S28277.

References

  • Adikaram NKB, Yakandawala DMD (2020) A checklist of plant pathogenic fungi and Oomycota in Sri Lanka. Ceylon Journal of Science 49:93–123

    Article  Google Scholar 

  • Ahad M, Islam M, Nupur N (2018) Effect of plant extracts on seed borne fungi of jute. American Journal of Plant Sciences 9:2580–2592

    Article  Google Scholar 

  • Ahmad S, Iqbal SH, Khalid AN. (1997) Fungi of Pakistan. Sultan Ahmad Mycological Society of Pakistan, Lahour

  • Alam MW, Rehman A, Malik AU, Khan SM, Ali S, Aslam S, Sarfraz S et al (2018) First report of Alternaria alternata causing postharvest fruit rot of jujube in Pakistan. Plant Disease 102:452–453

    Article  Google Scholar 

  • Alfieri SA Jr, Langdon KR, Wehlburg C, Kimbrough JW (1984) Index of Plant Diseases in Florida (Revised). Florida Department of Agriculture and Consumer Service, Division of Plant Industry, Bulletin 11:1–389

  • Alves JL, Woudenberg JHC, Durate LL, Crous PW, Barreto RW (2013) Reappraisal of the genus Alternariaster (Dothideomycetes). Persoonia 31:77–85

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Anonymous. (1979) List of plant diseases in Taiwan, Plant Protection Society, Republic of China, 404 pages

  • Ariyawansa HA, Hyde KD, Jayasiri SC, Buyck B, Chethana KWT, Dai DQ et al (2015) Fungal diversity notes 111–252–taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 75:27–274

    Article  Google Scholar 

  • Aveskamp MM, Woudenberg JHC, de Gruyter J, Turco E, Groenewald JZ, Crous PW (2009) Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification ingerprinting (DAF): a case study in the Phoma exigua species complex. Molecular Plant Pathology 10:403–414

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Aveskamp MM, de Gruyter J, Woudenberg JHC, Verkley GJM, Crous PW (2010) Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related pleosporalean genera. Studies in Mycology 65:1–60

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Beneke ES, Rogers AL (1996) Medical mycology and human mycoses. Star Publishing Company, Belmont, Tennessee

  • Boerema GH, De Gruyter J, Noordeloos ME (2004) Phoma identification manual. Differentiation of specific and infra- specific taxa in culture. CABI Publishing, USA

  • Chen Q, Jiang JR, Zhang GZ, Cai L, Crous PW (2015) Resolving the Phoma enigma. Studies in Mycology 82:137–217

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen Q, Hou LW, Duan WJ, Crous PW, Cai L (2017) Didymellaceae revisited. Studies in Mycology 87:105–159

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Crous PW, Wingfield MJ, Burgess TI, Hardy GEStJ, Gené J, Guarro J et al. (2018) Fungal Planet description sheets: 716–784. Persoonia 40: 240–393

  • Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9:772

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • De Gruyter J, Aveskamp MM, Woudenberg JHC, Verkley GJM, Groenewald JZ, Crous PW (2009) Molecular phylogeny of Phoma and allied anamorph genera: towards a reclassification of the Phoma complex. Mycological Research 113:508–519

    Article  PubMed  Google Scholar 

  • De Gruyter J, Noordeloos ME, Boerema GH (1998) Contributions towards amonograph of Phoma (Coelomycetes) – I. 3. Section Phoma: taxa withconidia longer than 7 μm. Persoonia 16:471–490.

  • De Gruyter J, Woudenberg JHC, Aveskamp MM, Verkley GJM, Groenewald JZ, Crous PW (2010) Systematic reappraisal of species in Phoma section Paraphoma, Pyrenochaeta and Pleurophoma. Mycologia 102:1066–1081

    Article  PubMed  Google Scholar 

  • De Gruyter J, Woudenberg JHC, Aveskamp MM, Verkley GJM, Groenewald JZ, Crous PW (2013) Redisposition of phoma-like anamorphs in Pleosporales. Studies in Mycology 75:1–36

    Article  PubMed  Google Scholar 

  • De Gruyter J (2012) Revised taxonomy of Phoma and allied genera. Ph.D. dissertation. Wageningen University, Netherlands

  • Dilmaghani A, Balesdent MH, Rouxel T, Moreno-Rico O (2010) First report of Leptosphaeria biglobosa (blackleg) on Brassica oleracea (cabbage) in Mexico. Plant Disease 94:791

    Article  CAS  PubMed  Google Scholar 

  • Edler D, Klein J, Antonelli A, Silvestro (2019) raxmlGUI 2.0 beta: a graphical interface and toolkit for phylogenetic analyses using RAxMl. bioRxiv.

  • Erwin DC, Ribeiro OK. (1996) Phytophthora Diseases worldwide. American Phytopathological Society, Saint Paul, Minnesota, USA

  • Farr DF, Rossman AY (2021) Fungal Databases: U.S. National Fungus Collections, ARS, USDA. Internet Resource: https://nt.ars-grin.gov/fungaldatabases/ (Retrieved April 12. 2021)

  • Gadgil PD (2005) Fungi on trees and shrubs in New Zealand. Vol. 4 Fungi of New Zealand. Fungal Diversity Press, Hong Kong

  • Golzar H, Lanoiselet V, Wang C, Tan YP, Shivas RG (2015) First report of Phoma multirostrata in Australia. Australasian Plant Disease Notes 10:8

    Article  Google Scholar 

  • Gupta PC, Madaan RL (1977) Fruit rot diseases of ber (Ziziphus mauritiana L.) from Haryana. Indian Phytopathology 30:554–555

    Google Scholar 

  • Gupta D, Razdan VK (2010) First report of Pestalotia funera causing leaf spot disease on Ziziphus mauritiana from India. Indian Phytopathology 63:240

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41:95–98

    CAS  Google Scholar 

  • Heidari K, Farokhinejad R, Mehrabi-Koushki M (2018) Occurrence of purslane leaf spot caused by Dichotomophthora lutea in Iran. Australasian Plant Disease Notes 13:33

    Article  Google Scholar 

  • Hilton S, Ed (2000) Canadian plant disease survey. Agriculture and Agri-Food Canada 80:151

  • Hou LW, Groenewald JZ, Pfenning LH, Yarden O, Crous PW, Cai L (2020a) The phoma-like dilemma. Studies in Mycology 96:309–396

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hou LW, Hernández-Restrepo M, Groenewald JZ, Cai L, Crous PW (2020b) Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes). MycoKeys 65:49–99

    Article  PubMed  PubMed Central  Google Scholar 

  • Hyde KD, Hongsanan S, Jeewon R, Bhat DJ, McKenzie EHC, Gareth Jones EB et al (2016) Fungal diversity notes 367–490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80:1–270

    Article  Google Scholar 

  • Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Gareth Jones EB et al (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 100:5–277

    Article  Google Scholar 

  • Jamadar MM, Balikai RA, Sataraddi AR (2009) Status of diseases on ber (Ziziphus mauritiana lamarck) in India and their management options. Acta Horticulturae 840:383–390

    Article  CAS  Google Scholar 

  • Khokhriakov (1933) Diseases pests of new cultivated textile plants. Inst. New Bast Raw Material Vaskhnil, Moscow. pp. 61–67

  • Larki R, Mehrabi-Koushki M, Farokhinejad R (2019) Ectophoma iranica sp. nov. and new hosts and records of Allophoma spp. in Iran. Journal of Phytopathology 167:538–545

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16:1799–1808

    Article  CAS  PubMed  Google Scholar 

  • Lob S, Jaspers MV, Ridgway HJ, Jones EE (2013) First report of Leptosphaeria biglobosa as a stem canker pathogen of Brassicas in New Zealand. Plant Disease 97:1113

    Article  CAS  PubMed  Google Scholar 

  • Machowicz-stefaniak Z, Zimowska B, Zalewska E (2008) The occurence and pathogenicity of Phoma exigua Desm. var. exigua for selected species of herbs. Acta Agrobotanica 61:157–166

  • Mendes MAS, da Silva VL, Dianese JC, Ferreira MASV, dos Santos CEN, Gomes NE et al. (1998) Fungos em plants no Brasil. Brasil, Embrapa-SPI/Embrapa-Cenargen, Brasilia

  • O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB International, Wallingford, pp 225–233

    Google Scholar 

  • Orieux L, Felix S (1968) List of plant diseases in Mauritius. Phytopathology 7:1–48

    Google Scholar 

  • Pennycook SR (1989) Plant diseases recorded in New Zealand. Vol. 3. Auckland, Plant Disease Division. D.S.I.R.

  • Phukhamsakda C, McKenzie EHC, Phillips AJL, Gareth Jones EB, Bhat DJ, Stadler M et al (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102:1–203

    Article  Google Scholar 

  • Priest MJ (2006) Fungi of Australia. Septoria. Australian Biological Resources Study/ CSIRO Publishing, Melbourne

  • Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1:17–20

    Article  CAS  Google Scholar 

  • Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S et al (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539–542

    Article  PubMed  PubMed Central  Google Scholar 

  • Safi A, Mehrabi-Koushki M, Farokhinejad R (2020) Amesia khuzestanica and Curvularia iranica spp. nov. from Iran. Mycological Progress 19:935–945

  • Sawada K (1959) Descriptive catalogue of Taiwan (Formosan) fungi. XI. Special Publications, College of Agriculture, National Taiwan University 8:1–268

  • Shaw CG (1973) Host fungus index for the Pacific Northwest - I. Hosts. Washington Agricultural Experimental Station, Bulletin

  • Shivas RG (1989) Fungal and bacterial diseases of plants in Western Australia. Journal of the Royal Society of Western Australia 72:1–62

    Google Scholar 

  • Sung GH, Sung JM, Hywel-Jones NL, Spatafora JW (2007) A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a combinational bootstrap approach. Molecular Phylogenetics and Evolution 44:1204–1223

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thaung MM (2008) Biodiversity survey of coelomycetes in Burma. Australasian Mycologist 27:74–110

    Google Scholar 

  • Thompson A, Johnston A (1953) A host list of plant diseases in Malaya. Mycological Papers 52:1–38

    Google Scholar 

  • Valenzuela-Lopez N, Cano-Lira JF, Guarro J, Sutton DA, Wiederhold N, Crous PW, Stchigel AM (2018) Coelomycetous Dothideomycetes with emphasis on the families Cucurbitariaceae and Didymellaceae. Studies in Mycology 90:1–69

    Article  CAS  PubMed  Google Scholar 

  • Vanev SG, Sameva EF, Bakalova GG (1997) Order Sphaeropsidales Fungi Bulgaricae 3:1–335

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: InnisMA,Gelfand DH, Sninsky JJ,White TJ (Eds) PCR protocols: a guide to methods and applications. Academic Press, New York. pp. 315–322

  • Woudenberg JHC, Aveskamp MM, de Gruyter J, Spiers AG, Crous PW (2009) Multiple Didymella teleomorphs are linked to the Phoma clematidina morphotype. Persoonia 22:56–62

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

This work was financially supported by grant (SCU.AP99.294) from Research Council of Shahid Chamran University of Ahvaz.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran

    S. Akram Ahmadpour, Mehdi Mehrabi-Koushki & Reza Farokhinejad

  2. Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mehdi Mehrabi-Koushki

  3. Department of Botany, Agricultural Research, Education and Extension Organization (AREEO), Iranian Research Institute of Plant Protection, Tehran, Iran

    Bita Asgari

Authors
  1. S. Akram Ahmadpour
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  2. Mehdi Mehrabi-Koushki
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  3. Reza Farokhinejad
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  4. Bita Asgari
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Contributions

S. Akram Ahmadpour carried out sample preparation, fungal isolation and purification, pathogenicity experiments, morphometric and morphological determination, and DNA isolation and amplification. Mehdi Mehrabi-Koushki carried out the design and implementation of the research, DNA and phylogenetic analysis, and the writing of the manuscript. Two other authors, Reza Farokhinejad and Bita asgari, contributed to the implementation of the research and the revising of the manuscript.

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Correspondence to Mehdi Mehrabi-Koushki.

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Ahmadpour, S.A., Mehrabi-Koushki, M., Farokhinejad, R. et al. Xenodidymella iranica sp. nov. and new hosts of X. glycyrrhizicola in Iran. Trop. plant pathol. 47, 430–441 (2022). https://doi.org/10.1007/s40858-022-00491-3

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