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Sooty leaf spot—a new fungal disease of sorghum caused by Paraphaeosphaeria sorghi sp. nov

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Abstract

A leaf spot disease—herein named sooty leaf spot of sorghum (Sorghum bicolor)—was observed in Viçosa, Minas Gerais, Brazil. A pycnidial fungus producing fuliginous conidial masses was regularly found associated with sooty spots on leaf blades and sheaths. The fungus was isolated in pure culture from specimens collected at two localities. The examination of morphological features combined with molecular analysis of the internal transcribed spacer (ITS) region of rDNA, large subunit ribosomal ribonucleic acid (LSU rDNA) and β-tubulin region (BenA) clearly demonstrated that the two isolates were grouped in a single clade, separate from other Paraphaeosphaeria species, a new taxon. Inoculations of both isolates of the new species on maize, sugarcane and sorghum plants led to disease development only on sorghum; therefore, Koch’s postulates were fulfilled for this fungus–host association. Here, we describe the new species Paraphaeosphaeria sorghi sp. nov. as a novel fungus causing leaf spot of S. bicolor in Brazil and worldwide and represent an addition to the long list of fungal diseases of sorghum.

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Data availability statement

The datasets generated during and analyzed during the current study are available in the GenBank repository (http://www.ncbi.nlm.nih.gov/genbank).

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Acknowledgements

We thank Dr. Adans A. Cólman for providing us with one of the isolates used in this study and preliminarily examining the fungus. This study received financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil

    Letícia Rosa Vieira de Paula, Robert Weingart Barreto & Thaisa Ferreira da Nóbrega

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  1. Letícia Rosa Vieira de Paula
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  2. Robert Weingart Barreto
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  3. Thaisa Ferreira da Nóbrega
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Contributions

Letícia Rosa Vieira de Paula took part in methodology, investigation, validation. Robert Weingart Barreto involved in conceptualization, original collection, supervision, writing review & editing. Thaisa Ferreira da Nóbrega involved in writing—original draft, preparation, supervision.

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Correspondence to Thaisa Ferreira da Nóbrega.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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de Paula, L.R.V., Barreto, R.W. & da Nóbrega, T.F. Sooty leaf spot—a new fungal disease of sorghum caused by Paraphaeosphaeria sorghi sp. nov. Trop. plant pathol. 47, 618–625 (2022). https://doi.org/10.1007/s40858-022-00522-z

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