Abstract
Anthracnose of grapes inflicted by Sphaceloma ampelinum (de Bary) is widespread and the most destructive disease of grapes (Vitis vinifera L.) worldwide. The pathogen produced irregular, whitish glabrous raised colony which later turned brownish white and developed concentric rings. Acervulus (62.05–79.01 μm) developed in concentric rings were light brown, round to flask shaped bearing hyaline to greenish, guttulated, circular to oblong conidia (5.19 × 2.97 µm) after 20 days of incubation at 28 ± 2 °C. To confirm the morphological identification, the rDNA of isolated pathogen was amplified with 18 s RNA primers to give the expected amplicon of 500 bp.The best culture media for surface mycelium growth was oat meal agar and corn meal agar was found as best media for sporulation of the pathogen. Whereas, maximum mycelia growth and sporulation was achieved at pH 6.5 and temperature of 25–30 °C. Among the bio-control agents screened, Trichoderma harzianum, exhibited maximum inhibition in mycelial growth whereas, among the new fungicides tested, combination of systemic fungicides viz., carbendazim (25%) + flusilazole (12.5%) 37.5 SC @ 0.1%, carbendazim (12%) + mancozeb (63%) 75 WP @ 0.25% and metiram (55%) + pyraclostrobin (5%) @ 0.1% proved effective for the management of the disease.
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The financial support provided by Head of Division, Plant Pathology SKUAST-Kashmir for this research work is acknowledged.
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Fayaz, U., Banday, S., Shahnaz, E. et al. Morpho-cultural, physiological and molecular characterisation of Sphaceloma ampelinum causing anthracnose of grapes in temperate region of India and its management. Indian Phytopathology 74, 949–957 (2021). https://doi.org/10.1007/s42360-021-00408-1
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DOI: https://doi.org/10.1007/s42360-021-00408-1