Abstract
The jackfruit tree (Artocarpus heterophyllus Lam.) is one of the most important fruit crops and is widely cultivated in Vietnam. The surveys conducted in Dak Lak province in 2022 revealed that fruit rot showing dark brown to black lesions was a serious disease of mature jackfruit in Dak Lak province, Vietnam. A causative agent was isolated from 26 diseased samples and identified as Lasiodiplodia theobromae based on morphological characteristics and multi-locus analyses of ITS, TEF1-α and TUB. Its pathogenicity was confirmed by artificial inoculation. To our knowledge, this is the first report of L. theobromae causing fruit rot of jackfruit in Vietnam.
Data availability
The DNA sequence data that support the findings of this study are available in NCBI https://www.ncbi.nlm.nih.gov/.
References
Adikaram N, Manawadu L, Jayasinghe L, Yakandawala D (2020) First report of Lasiodiplodia theobromae rot in ripe jackfruit (Artocarpus heterophyllus Lam.) in Sri Lanka. Indian Phytopathol 73:583–585. https://doi.org/10.1007/s42360-020-00243-w
Carbone I, Kohn L (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556
General statistics office (2022) Achievements of the agriculture industry—a year summation. Statistical Publishing House, Hanoi
Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330
Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW (2013) The Botryosphaeriaceae: genera and species known from culture. Stud Mycol 76:51–167
White TJ, Bruns TD, Lee S, Taylor S (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols—a guide to methods and applications. Elsevier, Amsterdam, pp 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Funding
This present research was financially supported by Department of Science and Technology, Dak Lak, Vietnam (Grant number: 364/HD-SKHCB).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Thao, L.D., Hien, L.T., Trang, T.T.T. et al. First report of Lasiodiplodia theobromae causing fruit rot of jackfruit in Vietnam. Indian Phytopathology 76, 1137–1140 (2023). https://doi.org/10.1007/s42360-023-00684-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s42360-023-00684-z