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Effects of spores and raw entomotoxins from Beauveria bassiana BMAUM-M6004 on Tuta absoluta (Lepidoptera: Gelechiidae)

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Abstract

Tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is considerably an important tomato pests. Eco-friendly plant protection products are very prominent in minimizing the current intensive use of synthetic pesticides. The aim of this study was to evaluate the effectiveness spectrum of the local isolate of B bassiana BMAUM-M6004 against T. absoluta larvae by testing conidiospores, blastospores and crude solutions of extracellular enzymes and secondary metabolites. BMAUM-M6004 showed an overall significant effect on all larval stages of T. absoluta with mortalities and median lethal times being dependent upon extract types and concentrations. On 11th day post treatment, conidia were caused relatively high mortalities of 93.33%, 86.67% and 91.17% on the 1st, 2nd and 3rd instar larvae, respectively, while blastospores brought about 95.56% and 93.33% of mortalities on the 3rd and 4th instar larvae, respectively. For metabolic toxins: raw extracellular enzymes induced at least 73.33% mortality, while the highest mortalities resulted in secondary metabolites were 73.33% and 78.67% on the 1st and 2nd instar larvae, respectively. In general, extracellular enzymes were found more effective as mixtures than as separate treatments. For instance, the highest mortality (91.66%) was obtained on the 10th day post treatments for the mixture of lipase, chitinase and protease in 2:1:1 ratio, followed by the mixture of the same enzymes in 2:2:1 ratio with 88.33% mortality. B. bassiana BMAUM-M6004 was found to be a potential agent for the control of tomato leaf miner T. absoluta in different formulations.

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Data Availability

JC NDAYIRAGIJE, T ÖZEK and İ KARACA implemented this research and state that all presented data and material are available.

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Acknowledgements

Gursel KARACA1 provided laboratory infrastructures and mentorship for the research implementation.

Funding

This research was financially supported by the Department of Scientific Research Projects of Isparta University of Applied Sciences (BAP) with the project name of “Beauveria bassiana’nın farklı spor ve metabolitlerinin Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) larvalarına etkisi” [In Turkish] and [2021-YL1-0142] as a project number.

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  1. Plant Protection Department, Faculty of Agriculture, Isparta University of Applied Sciences, 32260, Isparta, Turkey

    Jean Claude Ndayiragije, Tuğçe Özek & İsmail Karaca

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  1. Jean Claude Ndayiragije
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  3. İsmail Karaca
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JC NDAYIRAGIJE participated in experimentation and writing, T ÖZEK participated in experimentation (100/2000 CoHE PhD Scholarship) and İ KARACA participated in data analyses and with financial supports.

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Correspondence to Jean Claude Ndayiragije.

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JC NDAYIRAGIJE, T ÖZEK and İ KARACA declare that they have no conflicts of interests.

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Ndayiragije, J.C., Özek, T. & Karaca, İ. Effects of spores and raw entomotoxins from Beauveria bassiana BMAUM-M6004 on Tuta absoluta (Lepidoptera: Gelechiidae). Int J Trop Insect Sci 43, 1783–1795 (2023). https://doi.org/10.1007/s42690-023-01091-7

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