Skip to main content
SpringerLink
Log in

Yarrowia lipolytica: An Overview

  • Chapter
  • First Online:
Biotechnological Applications of the Yeast Yarrowia lipolytica

Part of the book series: SpringerBriefs in Microbiology ((BRIEFSMICROBIOL))

Abstract

The nonconventional yeast Yarrowia lipolytica is an interest for fundamental research and biotechnological applications. The fundamental studies play a crucial role in the establishment and development of the biotechnological processes. Hence, this chapter will give an overview about Y. lipolytica fundamental studies including taxonomy, ecology, morphology, cell biology, physiology, metabolism, genetics, and molecular biology. This yeast is currently used as a model for the study of dimorphism, peroxisome biogenesis, degradation of hydrophobic substrates, protein secretion, and several new fields.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Amaral PFF, da Silva JM, Lehocky M, Barros-Timmons AMV, Coelho MAZ, Marrucho IM, Coutinho JAP (2006a) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Process Biochem 41(8):1894–1898

    Article  CAS  Google Scholar 

  • Amaral PFF, de Almeida APR, Peixoto T, Rocha-Leao MHM, Coutinho JAP, Coelho MAZ (2007) Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production. World J Microbiol Biotechnol 23(3):339–344

    Article  CAS  Google Scholar 

  • Amaral PFF, Freire MG, Rocha-Leao MHM, Marrucho IM, Coutinho JAP, Coelho MAZ (2008) Optimization of oxygen mass transfer in a multiphase bioreactor with perfluorodecalin as a second liquid phase. Biotechnol Bioeng 99(3):588–598

    Article  PubMed  CAS  Google Scholar 

  • Amaral PFF, Rocha-Leao MHM, Marrucho IM, Coutinho JAP, Coelho MAZ (2006b) Improving lipase production using a perfluorocarbon as oxygen carrier. J Chem Technol Biotechnol 81(8):1368–1374

    Article  CAS  Google Scholar 

  • Andrade MJ, Rodriguez M, Sanchez B, Aranda E, Cordoba JJ (2006) DNA typing methods for differentiation of yeasts related to dry-cured meat products. Int J Food Microbiol 107(1):48–58

    Article  PubMed  CAS  Google Scholar 

  • Barth G, Gaillardin C (1996) Yarrowia lipolytica. In: Wolf K (ed) Nonconventional yeasts in biotechnology. Springer, Berlin, pp 313–388. doi:10.1007/978-3-642-79856-6_10

  • Barth G, Gaillardin C (1997) Physiology and genetics of the dimorphic fungus Yarrowia lipolytica. FEMS Microbiol Rev 19(4):219–237

    Article  PubMed  CAS  Google Scholar 

  • Barth G, Künkel W (1979) Alcohol dehydrogenase (ADH) in yeasts. II. NAD+-and NADP+-dependent alcohol dehydrogenases in Saccharomycopsis lipolytica. Z Allg Mikrobiol 19(6):381–390

    Article  PubMed  CAS  Google Scholar 

  • Barth G, Weber H (1985) Improvement of sporulation in the yeast Yarrowia lipolytica. Antonie Van Leeuwenhoek 51(2):167–177. doi:10.1007/BF02310010

    Article  PubMed  CAS  Google Scholar 

  • Beckerich JM, Baudevin AB, Gaillardin C (1998) Yarrowia lipolytica: a model organism for protein secretion studies. Int Microbiol 1:123–130

    PubMed  CAS  Google Scholar 

  • Biryukova EN, Medentsev AG, Arinbasarova AY, Akimenko VK (2006) Tolerance of the yeast Yarrowia lipolytica to oxidative stress. Microbiol 75(3):243–247

    Article  CAS  Google Scholar 

  • Casaregola S, Feynerol C, Diez M, Fournier P, Gaillardin C (1997) Genomic organization of the yeast Yarrowia lipolytica. Chromosoma 106(6):380–390

    Article  PubMed  CAS  Google Scholar 

  • Casaregola S, Neuvéglise C, Bon E, Gaillardin C (2002) Ylli, a Non–LTR retrotransposon L1 family in the dimorphic yeast Yarrowia lipolytica. Mol Biol Evol 19(5):664–677

    Article  PubMed  CAS  Google Scholar 

  • Casaregola S, Neuveglise C, Lepingle A, Bon E, Feynerol C, Artiguenave F, Wincker P, Gaillardin C (2000) Genomic exploration of the hemiascomycetous Yeasts: 17. Yarrowia lipolytica. FEBS Lett 487(1):95–100

    Article  PubMed  CAS  Google Scholar 

  • Chang C-F, Chen C-C, Lee C-F, Liu S-M (2013) Identifying and characterizing Yarrowia keelungensis sp. nov., an oil-degrading yeast isolated from the sea surface microlayer. Antonie Van Leeuwenhoek 104(6):1117–1123. doi:10.1007/s10482-013-0033-z

    Article  PubMed  CAS  Google Scholar 

  • Chi Z, Wang F, Wang L, Li J, Wang X (2007) Selection of Yarrowia lipolytica strains with high protein content from yeasts isolated from different marine environments. J Ocean Univ China 6(4):360–364. doi:10.1007/s11802-007-0360-7

    Article  CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1984) Isolation of a bioemulsifier from Candida lipolytica. Appl Environ Microbiol 48(4):747–750

    PubMed Central  PubMed  CAS  Google Scholar 

  • Cirigliano MC, Carman GM (1985) Purification and characterization of Liposan, a Bioemulsifier from Candida lipolytica. Appl Environ Microbiol 50(4):846–850

    PubMed Central  PubMed  CAS  Google Scholar 

  • Coelho MAZ, Amaral PFF, Belo I (2010) Yarrowia lipolytica: an industrial workhorse. In: Méndez-Villas A (ed) Current research, technology and education topics in applied microbiology and microbial biotechnology, vol 2, 2nd edn. Formatex, Badajoz, pp 930–944

    Google Scholar 

  • Deak T, Chen J, Beuchat LR (2000) Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry. Appl Environ Microbiol 66(10):4340–4344

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Dell’Angelica EC, Stella CA, Ermácora MR, Ramos EH, Santome J (1992) Study on fatty acid binding by proteins in yeast. Dissimilar results in Saccharomyces cerevisiae and Yarrowia lipolytica. Comp Biochem Physiol B Comp Biochem 102(2):261–265. doi:http://dx.doi.org/10.1016/0305-0491(92)90120-G

  • Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, de Montigny J, Marck C, Neuveglise C, Talla E, Goffard N, Frangeul L, Aigle M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich J-M, Beyne E, Bleykasten C, Boisrame A, Boyer J, Cattolico L, Confanioleri F, de Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, Groppi A, Hantraye F, Hennequin C, Jauniaux N, Joyet P, Kachouri R, Kerrest A, Koszul R, Lemaire M, Lesur I, Ma L, Muller H, Nicaud J-M, Nikolski M, Oztas S, Ozier-Kalogeropoulos O, Pellenz S, Potier S, Richard G-F, Straub M-L, Suleau A, Swennen D, Tekaia F, Wesolowski-Louvel M, Westhof E, Wirth B, Zeniou-Meyer M, Zivanovic I, Bolotin-Fukuhara M, Thierry A, Bouchier C, Caudron B, Scarpelli C, Gaillardin C, Weissenbach J, Wincker P, Souciet J-L (2004) Genome evolution in yeasts. Nature 430(6995):35–44

    Article  PubMed  Google Scholar 

  • El-Sherbeini M, Bostia KA, Levitr J, Mitchel D (1987) Gene-protein assignments within the yeast Yarrowia lipolytica dsRNA viral genome. Curr Genet 11(6–7):483–490. doi:10.1007/BF00384610

    Article  PubMed  CAS  Google Scholar 

  • Encinas JP, Lopez-Diaz TM, Garcia-Lopez ML, Otero A, Moreno B (2000) Yeast populations on Spanish fermented sausages. Meat Sci 54(3):203–208

    Article  PubMed  CAS  Google Scholar 

  • Fickers P, Benetti PH, Wache Y, Marty A, Mauersberger S, Smit MS, Nicaud JM (2005) Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res 5(6–7):527–543

    Article  PubMed  CAS  Google Scholar 

  • Flores C-L, Rodríguez C, Petit T, Gancedo C (2000) Carbohydrate and energy-yielding metabolism in non-conventional yeasts1. FEMS Microbiol Rev 24(4):507–529. doi:10.1111/j.1574-6976.2000.tb00553.x

    Article  PubMed  CAS  Google Scholar 

  • Fournier P, Gaillardin C, Persuy M-A, Klootwijk J, Heerikhuizen Hv (1986) Heterogeneity in the ribosomal family of the yeast Yarrowia lipolytica: genomic organization and segregation studies. Gene 42(3):273–282. doi:http://dx.doi.org/10.1016/0378-1119(86)90231-3

    Google Scholar 

  • Gardini F, Suzzi G, Lombardi A, Galgano F, Crudele MA, Andrighetto C, Schirone M, Tofalo R (2001) A survey of yeasts in traditional sausages of southern Italy. FEMS Yeast Res 1(2):161–167

    Article  PubMed  CAS  Google Scholar 

  • Gardini F, Tofalo R, Belletti N, Iucci L, Suzzi G, Torriani S, Guerzoni ME, Lanciotti R (2006) Characterization of yeasts involved in the ripening of Pecorino Crotonese cheese. Food Microbiol 23(7):641–648

    Article  PubMed  CAS  Google Scholar 

  • Gellissen G, Kunze G, Gaillardin C, Cregg JM, Berardi E, Veenhuis M, van der Klei I (2005) New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica—A comparison. FEMS Yeast Res 5(11):1079–1096

    Article  PubMed  CAS  Google Scholar 

  • Hassanshahian M, Tebyanian H, Cappello S (2012) Isolation and characterization of two crude oil-degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32, from the Persian Gulf. Mar Pollut Bull 64(7):1386–1391. doi:http://dx.doi.org/10.1016/j.marpolbul.2012.04.020

  • Heslot H (1990) Genetics and genetic engineering of the industrial yeast Yarrowia lipolytica. Applied molecular genetics. Springer, Berlin, pp 43–73

    Chapter  Google Scholar 

  • Jacques N, Casaregola S (2008) Safety assessment of dairy microorganisms: the hemiascomycetous yeasts. Int J Food Microbiol 126(3):321–326. doi:http://dx.doi.org/10.1016/j.ijfoodmicro.2007.08.020

    Google Scholar 

  • Juretzek T, Le Dall MT, Mauersberger S, Gaillardin C, Barth G, Nicaud JM (2001) Vectors for gene expression and amplification in the yeast Yarrowia lipolytica. Yeast 18(2):97–113

    Article  PubMed  CAS  Google Scholar 

  • Kerscher S, Durstewitz G, Casaregola S, Gaillardin C, Brandt U (2001) The complete mitochondrial genome of Yarrowia lipolytica. Comp Funct Genomics 2(2):80–90. doi:10.1002/cfg.72

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Kim J, Cheon SA, Park S, Song Y, Kim JY (2000a) Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica. FEMS Microbiol Lett 190(1):9–12

    Article  PubMed  CAS  Google Scholar 

  • Kim TH, Oh YS, Kim SJ (2000b) The possible involvement of the cell surface in aliphatic hydrocarbon utilization by an oil-degrading yeast, Yarrowia lipolytica 180. J Microbiol Biotechnol 10(3):333–337

    CAS  Google Scholar 

  • Klug MJ, Markovetz AJ (1967) Degradation of hydrocarbons by members of the genus candida II. Oxidation of n-Alkanes and 1-Alkenes by Candida lipolytica. J Bacteriol 93(6):1847–1852

    PubMed Central  PubMed  CAS  Google Scholar 

  • Kohlwein SD, Paltauf F (1984) Uptake of fatty acids by the yeasts, Saccharomyces uvarum and Saccharomycopsis lipolytica. Biochim Biophys Acta 792:310–317

    Article  PubMed  CAS  Google Scholar 

  • Kurtzman CP, Fell JW, Boekhout T (eds) (2011) The yeasts a taxonomic study, 5th edn. Elsevier, London. doi:http://dx.doi.org/10.1016/B978-0-444-52149-1.00196-8

  • Lachance M-A (2006) Yeast biodiversity: how many and how much? In: Péter G, Rosa C (eds) Biodiversity and ecophysiology of yeasts. The yeast handbook. Springer, Berlin, pp 1–9. doi:10.1007/3-540-30985-3_1

  • Lopandic K, Zelger S, Banszky LK, Eliskases-Lechner F, Prillinger H (2006) Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiol 23(4):341–350

    Article  PubMed  CAS  Google Scholar 

  • Lopez MC, Nicaud JM, Skinner HB, Vergnolle C, Kader JC, Bankaitis VA, Gaillardin C (1994) A phosphatidylinositol/phosphatidylcholine transfer protein is required for differentiation of the dimorphic yeast Yarrowia lipolytica from the yeast to the mycelial form. J Cell Biol 125(1):113–127. doi:10.1083/jcb.125.1.113

    Article  PubMed  CAS  Google Scholar 

  • Mafakher L, Mirbagheri M, Darvishi F, Nahvi I, Zarkesh-Esfahani H, Emtiazi G (2010) Isolation of lipase and citric acid producing yeasts from agro-industrial wastewater. New Biotechnol 27(4):337–340. doi:10.1016/j.nbt.2010.04.006

    Article  CAS  Google Scholar 

  • Matsuoka M, Matsubara M, Daidoh H, Imanaka T, Uchida K, Aiba S (1993) Analysis of regions essential for the function of chromosomal replicator sequences from Yarrowia lipolytica. Molec Gen Genet 237(3):327–333. doi:10.1007/BF00279435

    PubMed  CAS  Google Scholar 

  • Mekouar M, Blanc-Lenfle I, Ozanne C, Da Silva C, Cruaud C, Wincker P, Gaillardin C, Neuveglise C (2010) Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts. Genome Biol 11(6):R65

    Article  PubMed Central  PubMed  Google Scholar 

  • Mirbagheri M, Nahvi I, Emtiazi G, Mafakher L, Darvishi F (2012) Taxonomic characterization and potential biotechnological applications of Yarrowia lipolytica isolated from meat and meat products. Jundishapur J Microbiol 5(1):346–351. doi:10.5812/kowsar.20083645.2433

    Google Scholar 

  • Murphy GL, Perry JJ (1984) Assimilation of chlorinated alkanes by hydrocarbon-utilizing fungi. J Bacteriol 160(3):1171–1174

    PubMed Central  PubMed  CAS  Google Scholar 

  • Nagy E, Niss M, Dlauchy D, Arneborg N, Nielsen DS, Péter G (2013) Yarrowia divulgata f.a., sp. nov., a yeast species from animal-related and marine sources. Int J Syst Evol Microbiol 63(12):4818–4823. doi:10.1099/ijs.0.057208-0

  • Nicaud J-M (2012) Yarrowia lipolytica. Yeast 29(10):409–418. doi:10.1002/yea.2921

    Article  PubMed  CAS  Google Scholar 

  • Nuttley WM, Brade AM, Eitzen GA, Glover JR, Aitchison JD, Rachubinski RA, Gaillardin C (1993) Rapid identification and characterization of peroxisomal assembly mutants in Yarrowia lipolytica. Yeast 9(5):507–517. doi:10.1002/yea.320090506

    Article  CAS  Google Scholar 

  • Ogrydziak D, Bassel J, Contopoulou R, Mortimer R (1978) Development of genetic techniques and the genetic map of the yeast Saccharomycopis lipolytica. Molec Gen Genet 163(3):229–239. doi:10.1007/BF00271953

    Article  Google Scholar 

  • Papanikolaou S, Aggelis G (2003) Selective uptake of fatty acids by the yeast Yarrowia lipolytica. Eur J Lipid Sci Technol 105(11):651–655

    Article  CAS  Google Scholar 

  • Perez-Campo FM, Dominguez A (2001) Factors affecting the morphogenetic switch in Yarrowia lipolytica. Curr Microbiol 43(6):429–433

    Article  PubMed  CAS  Google Scholar 

  • Poritz MA, Siegel V, Hansen W, Walter P (1988) Small ribonucleoproteins in Schizosaccharomyces pombe and Yarrowia lipolytica homologous to signal recognition particle. Proc Natl Acad Sci 85(12):4315–4319

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Rodrigues G, Pais C (2000) The influence of acetic and other weak carboxylic acids on growth and cellular death of the yeast Yarrowia lipolytica. Food Technol Biotechnol 38(1):27–32

    CAS  Google Scholar 

  • Rosas-Quijano R, Gaillardin C, Ruiz-Herrera J (2008) Functional analysis of the MATB mating-type idiomorph of the dimorphic fungus Yarrowia lipolytica. Curr Microbiol 57(2):115–120. doi:10.1007/s00284-008-9162-4

    Article  PubMed  CAS  Google Scholar 

  • Ruiz-Herrera J, Sentandreu R (2002) Different effectors of dimorphism in Yarrowia lipolytica. Arch Microbiol 178(6):477–483

    Article  PubMed  CAS  Google Scholar 

  • Sanz A, Martin R, Mayoral MB, Hernandez PE, Gonzalez I, Lacarra TG (2005) Development of a PCR-culture technique for rapid detection of yeast species in vacuum packed ham. Meat Sci 71(2):230–237

    Article  PubMed  CAS  Google Scholar 

  • Scheller U, Zimmer T, Becher D, Schauer F, Schunck W-H (1998) Oxygenation cascade in conversion of n-Alkanes to alpha, omega-dioic acids catalyzed by cytochrome P450 52A3. J Biol Chem 273(49):32528–32534. doi:10.1074/jbc.273.49.32528

    Article  PubMed  CAS  Google Scholar 

  • Snow R (1966) An enrichment method for auxotrophic yeast mutants using the antibiotic ‘nystatin’. Nature 211(5045):206–207

    Article  PubMed  CAS  Google Scholar 

  • Spencer JFT, de Spencer ALR, Laluce C (2002) Non-conventional yeasts. Appl Microbiol Biotechnol 58(2):147–156

    Article  PubMed  CAS  Google Scholar 

  • Swennen D, Beckerich JM (2007) Yarrowia lipolytica vesicle-mediated protein transport pathways. BMC Evol Biol 7(219):1–19

    Google Scholar 

  • Tréton B, Dall M-T, Heslot H (1985) Virus-like particles from the yeast Yarrowia lipolytica. Curr Genet 9(4):279–284. doi:10.1007/BF00419956

    Article  Google Scholar 

  • van Heerikhuizen H, Ykema A, Klootwijk J, Gaillardin C, Ballas C, Fournier P (1985) Heterogeneity in the ribosomal RNA genes of the yeast Yarrowia lipolytica; cloning and analysis of two size classes of repeats. Gene 39(2–3):213–222

    Article  PubMed  Google Scholar 

  • Vasdinyei R, Deak T (2003) Characterization of yeast isolates originating from Hungarian dairy products using traditional and molecular identification techniques. Int J Food Microbiol 86(1–2):123–130

    Article  PubMed  CAS  Google Scholar 

  • Vernis L, Abbas A, Chasles M, Gaillardin CM, Brun C, Huberman JA, Fournier P (1997) An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica. Mol Cell Biol 17(4):1995–2004

    PubMed Central  PubMed  CAS  Google Scholar 

  • Zinjarde SS, Pant A (2002) Emulsifier from a tropical marine yeast, Yarrowia lipolytica NCIM 3589. J Basic Microbiol 42(1):67–73

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Microbiology, Department of Biology, University of Maragheh, Maragheh, Iran

    Farshad Darvishi Harzevili

Authors
  1. Farshad Darvishi Harzevili
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Farshad Darvishi Harzevili .

Rights and permissions

Reprints and permissions

Copyright information

© 2014 The Author(s)

About this chapter

Cite this chapter

Darvishi Harzevili, F. (2014). Yarrowia lipolytica: An Overview. In: Biotechnological Applications of the Yeast Yarrowia lipolytica. SpringerBriefs in Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-06437-6_1

Download citation

Publish with us

Policies and ethics

Search

Navigation