A study is made of nuclear division in Nematospora coryli, a pathogenic yeast. The DNA of cells (grown on a V-8-medium) was stained with leuco-basic fuchsin (Feulgen test) at pH 3.5. After budding has started the rounded nucleus elongates and some differentiation into chromosomes is perceptible. A few slides suggest the number of chromosomes being 4. After some time the nucleus appears to have duplicated. This nucleus migrates towards the isthmus between mother cell and bud. In the isthmus, or just in front of it, the two daughter nuclei proceed to disjoin and move along each other to opposite directions. One daughter nucleus moves into the bud; the other one migrates back into the mother cell.
Samples from synchronously growing cultures show that a fraction of the young yeast cells are destined to grow out to asci, in which after about 6 hours the presence of bivalents seems highly probable. The succeeding nuclear divisions take place in the same way as described for the vegetative cells and stop when the majority of the enlarged asci contain 8 nuclei.
Problems of haploidy and diploidy are discussed.
Small, densely stained bodies are observed in certain vegetative and some meiotic stages. As these bodies contain DNA, their function and possible homology with centrioles is discussed.
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References
Aist, J.R. (1969). The mitotic apparatus in fungi, Ceratocystis fagacearum and Fusarium oxysporum. J. Cell Biol. 40: 120–135.
Ashby, S.F. & W. Nowell (1926). The fungi of Stigmatomycosis. Ann. Bot. 40: 69–83.
Barry van Winkle, W., J.J. Biesele & R.P. Wagner (1971). The mitotic spindle apparatus of Neurospora crassa. Can. J. Genet. Cytol. 13: 873–887.
Berlin, J.D. & C.C. Bowen (1964). Centrioles in the fungus Albugo candida. Am. J. Bot. 51: 650–652.
Boveri, Th. (1895). Über das Verhalten der Centrosomen bei der Befruchtung des Seeigeleies nebst allgemeinen Bemerkungen über Centrosomen und Verwandtes. Verh. phys.-med. Ges. Würzb. 29: 1.
Carmo-Sousa, L. di (1970). Nematospora Peglion. In: The Yeasts, Lodder (ed.), pp. 440–447.
Colson, B. (1938). The cytology and development of Phyllactinea corylea Lev. Ann. Bot. N.S. 2: 381–402.
Du Praw, E.J. (1970). DNA and Chromosomes. Holt (ed.), Rinehart and Winston Inc. New York. pp. 58–70.
Duncan, E.J. & J.A. Macdonald (1967). Nuclear phenomena in Marasmius androsaceus (L. ex Fr.) Fr. and M. rotula (Scop. ex Fr.) Fr. Trans. R. Soc. Edinb. 66: 129–141.
Fulton, Ch. (1971). Centrioles. In: Origin and continuity of cell organelles. II. pp. 170–221. Springer Verlag, Berlin-Heidelberg.
Guilliermond, M.A. (1928). Recherches sur quelques Ascomycètes inférieurs isolés de la stigmatomycose des graines de cotonnier. Essai sur la phylogénie des Ascomycètes. Revue gén. Bot. 40: 555–576.
Harper, R.A. (1905). Sexual reproduction and the organization of the nucleus in certain middews. Publs Carnegie Instn 37: 1–104.
Hashimoto, T., S.F. Conti & H.B. Naylor (1959) Studies of the fine structure of microorganisms. IV. Observations on budding Saccharomyces cerevisiae. by light and electron microscopy. J. Bact. 77: 344–354.
Hejtmánková-Uhrová, N. & M. Hejtmánek (1968). Somatische Karyokinese bei Nannizia incurvata. Zentbl. Bakt. ParasitKde Abt., II 122: 185–196.
Hejtmánek, M. & N. Hejtmánková-Uhrová (1968). Vegetative nuclei and differentiation of chromosomes in Trichophyton vanbreuseghemii. Folia Microbiol. 13: 410–413.
Johnson, U.G. & K.R. Porter (1968). Fine structure of cell division in Chlamydomonas reinhardi. J. Cell Biol. 38: 403–425.
Laane, M.M. (1967). The nuclear division in Penicillium expansum. Can. J. Genet. Cytol. 9: 342–351.
Laane, M.M. (1969). Further cytological studies in the genus Penicillium Link. Hereditas 62: 158–184.
Leadbeater, B. & J.D. Dodge (1967). An electron microscope study of nuclear and cell division in a Dinoflagellate. Arch. Mikrobiol. 57: 239–254.
Leedale, G.F. (1968). In: The Biology of Euglena. Acad. Press, New York, p. 185.
Lodder J. & N.J.W. Kreger-van Rij (1967). Genus Nematospora Peglion. In: The Yeasts, a taxonomic study, pp. 324–331.
Lu, B.C. (1967). The course of meiosis and centriole behaviour during the ascus development of the Ascomycete Gelasinospora calospora. Chromosoma 22: 210–226.
Manuel, J. (1938). Sur la formation de l'asque de Nematospora coryli, après un phénomène sexuel. C. r. hebd. Séanc. Acad. Sci., Paris. 207: 1241–1243.
Matile, Ph., H. Moor & C.F. Robinow (1969). Yeast Cytology. In: The Yeasts, A.H. Rose & J.H. Harrison (eds.), pp. 219–302.
McClintock, B. (1945). Preliminary observations of the chromosomes of Neurospora crassa. Am. J. Bot. 32: 671–678.
McDonald, B.R. & J. Weijer (1966). The DNA content of centrioles of Neurospora crassa during division I and IV of Ascosporogenesis. Can. J. Genet. Cytol. 8: 42–50.
Moens, P.B. & E. Rapport, 1971. Spindles, spindle plaques and meiosis in the yeast Saccharomyces cerevisiae (Hansen). J. Cell Biol. 50: 344–361.
Moor, H. & K. Mühletaler (1963). Fine structure in frozenetched yeast cells. J. Cell Biol. 17: 609–628.
Namboodiri, A.N. & R.J. Lowry (1967). Vegetative nuclear division in Neurospora. Am. J. Bot. 54: 735–748.
Olive, L.S. (1953). The structure and behaviour of fungus nuclei. Bot. Rev. 19: 439–586.
Peglion, V. (1897). Patologia vegetale. Atti. d. Reali Accad. Lincei 6: 276–278.
Peglion, V. (1901). Ueber die Nematospora coryli Pegl. Zentbl. Bakt. ParasitKde II Abt., 7: 754–761.
Pontrefact, R.D. & J.J. Miller (1962). The metabolism of yeast sporulation. IV. Cytological and physiological changes in sporulating cells. Can. J. Microbiol. 8: 573–584.
Prahlada Rao, L.S. & A. Koopmans (1966). A spindle analogue in Saccharomyces cerevisiae Hansen. Experientia 22: 1–4.
Randall, J. & C. Disbrey (1965). Evidence for the presence of DNA at basal body sites in Tetrahymena pyriformis. Proc. R. Soc. Ser. B. 162: 473–491.
Rattner, J.B. & S.G. Phillips (1973). Independance of centriole formation and DNA synthesis. J. Cell Biol. 57: 359–372.
Robinow, C.F. (1961). Mitosis in the yeast Lipomyces lipofer. J. Cell Biol. 9: 879–892.
Robinow, C.F. & C.E. Caten (1969). Mitosis in Aspergillus nidulans. J. Cell Sci. 5: 403–431.
Robinow, C.F. & J. Marek (1966). A fiber apparatus in the nucleus of the yeast cell. J. Cell Biol. 29: 129–151.
Schneider, A. (1916). A parasitic Saccharomycete of the tomato. Phytopathology 6: 395–399.
Schopfer, W.H., D. Wustenfeld & G. Turian (1963). La division nucléaire chez Schizosaccharomyces pombe. Arch. Mikrobiol. 45: 304–313.
Seaman, G.R. (1960). Large-scale isolation of kinecosomes from the ciliated Protozoan Tetrahymena pyriformis. Exp. Cell Res. 21: 292–302.
Sillevis Smit, W.W. (1971). The yeast nucleus, ultrastructural organization and biosynthesis of ribosomal ribonucleic acid. Thesis, Utrecht.
Singleton, J.R. (1953). Chromosome morphology and the chromosome cycle in the ascus of Neurospora crassa. Am. J. Bot. 40: 124–144.
Smith-Sonneborn, J. & W. Plaut (1967). Evidence for the presence of DNA in the pellicle of Paramecium. J. Cell Sci. 2: 225–234.
Ward, E.W.B. & K.W. Ciurysek (1962). Somatic mitosis in Neurospora crassa. Am. J. Bot. 49: 393–399.
Weijer, J., A. Koopmans & D.L. Weijer (1963). Karyokinesis in vivo of the migrating somatic nucleus of Neurospora and Gelasinospora species. Trans. N. Y. Acad. Sci. II vol. 25: 846–854.
Weijer, J., A. Koopmans & D.L. Weijer (1965). Karyokinesis of somatic nuclei of Neurospora crassa. III. The juvenile and maturation cycles (Feulgen and Crystal Violet staining). Can. J. Genet. Cytol. 7: 140–163.
Weijer, J. & S.H. Weisberg (1966) Karyokinesis of the somatic nucleus of Aspergillus nidulans. I. The juvenile chromosome cycle (Feulgen staining). Can. J. Genet. Cytol. 8: 361–374.
Wells, K. (1970). Light and electron microscopic studies of Ascobolus, stercorarius, I. Nuclear division in the ascus. Mycologia 62: 761–790.
Wickerham, L.J., M.H. Flickinger & K.A. Burton (1946). A modification of Henrici's vegetable-juice sporulation medium for yeasts. J. Bact. 52: 611–612.
Williamson, D.H. (1964a). Techniques for synchronizing yeast cells. In: Synchrony in Cell Division and Growth, E. Zeuthen (ed.), Interscience Publ., New York.
Williamson, D.H. (1964b). Division Synchrony in Yeasts. In: Synchrony in Cell Division and Growth, E. Zeuthen (ed.), Interscience Publ., New York.
Williamson, D.H. (1966). Nuclear events in synchronously dividing yeast cultures. In: Cell synchrony. Studies in biosynthetic regulation. I.L. Cameron & G.M. Padula (eds.), Acad. Press, New York and London, pp. 81–101.
Williamson, D.H. & A.W. Scopes (1962). A rapid method for synchronizing division in the yeast, Saccharomyces cerevisiea. Nature 193: 256–257.
Wilson, C.L. & J.B. Aist (1967). Motility of fungal nuclei. Phytopathology 57: 769–771.
Wingard, S.A. (1925). Studies on the pathogenecity, morphology and cytology of Nematospora phaseoli. Bull. Torrey bot. Club 52: 249–290.
Winge, Ö & O. Laustsen (1940). On two types of spore germination and on genetic segregations in Saccharomyces, demonstrated through singlespore cultures. C. r. Trav. Lab. Carlsberg 22: 99–120.
Zickler, D. (1970). Division spindle and centrosomal plaques during mitosis and meiosis in some Ascomycetes. Chromosoma 30: 287–304.
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Koopmans, A. A cytological study of Nematospora coryli pegl. Genetica 47, 187–195 (1977). https://doi.org/10.1007/BF00123240
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DOI: https://doi.org/10.1007/BF00123240