| Authors | (Pat.) Griffon & Maubl. 1909 |
|---|---|
| Strain | 15006 |
| Classification | Botryosphaeriales, Botryosphaeriaceae, Lasiodiplodia |
| Culture collection | BCRC FU31238 |
| Detection frequency | Low |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Lasiodiplodia theobromae. a. Young conidia. b,d. Mature conidia. c. Conidiogenous cells and conidia. e. Paraphyses, conidiogenous cells and conidia (Bars= 5 μm, unless otherwise specified) |
| Colonies | Colonies on PDA attaining 63-mm at 25 °C after 5 days, sparsely cottony, initially white, turning dark olivaceous brown with age, margins entire, reverse similar, completely turning black in older culture. |
| Conidiomata | Conidiomata formed in culture after 3 wks, black, irregular, superficial or submerged. |
| Paraphyses | Paraphyses hyaline, cylindrical, slightly swollen near the base, 50–100(–140) × 1–2.5(–3.5) µm. |
| Conidiogenous cells | Conidiogenous cells hyaline, cylindrical, tapering toward the apex, 7–18 × 3–6 µm. |
| Conidia | Conidia ellipsoid with rounded ends, thick-walled, smooth, hyaline, aseptate, turning striated , dark brown and 1-septate when mature, 23–27 × 13–17 µm. |
| Note | This is a very common fungus causing diseases on diverse plant hosts and is also ubiquitous as saprobe. Accordingly it is not surprising for its occurring on rice grains. During this study, sporulation of this species can be promoted by adding a sterilized toothpick or filter paper onto the culture medium. |
| Pathogenicity | This species has been reported to cause root rot in rice (Claudius-Cole, 2018). On maize, it causes black kernal rot and death of seedlings via seed-borne inoculum. |
| Specimens examined | Taiwan, Pingtung County, rice grains, Jan 2014, Jie-Hao Ou, 14027 Taiwan, Taitung County, rice grains (cultivar Taiken 2), Jan 2015, Jie-Hao Ou, 15006 |
| ITS | CAGGTGGGACGTGCGTGAGACACATTACCGAGTTTTCGAGCTCCGGCTCGACTCTCCCACCCTTTGTGAACGTACCTCTGTTGCTTTGGCGGCTCCGGCCGCCAAAGGACCTTCAAACTCCAGTCAGTAAACGCAGACGTCTGATAAACAAGTTAATAAACTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTACAACCCTCAAGCTCTGCTTGGAATTGGGCACCGTCCTCACTGCGGACGCGCCTCAAAGACCTCGGCGGTGGCTGTTCAGCCCTCAAGCGTAGTAGAATACACCTCGCTTTGGAGCGGTTGGCGTCGCCCGCCGGACGAACCTTCTGAACTTTTCTCAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| EF1a | ACCGGTCGTAAGTTGCCCCTCACCTGCCTTGCGCCTCAAGGCAACATCGCTTACATGTTTATTAGACTTGATCTACAAGTGCGGCGGTATTGACAAGCGTACCATTGAGAAGTTCGAGAAGGTCCGTGCACGCATGTCGTTTTTTAACCCCTCTCGACTTCGGCGCTGCAGCGCTGCGGCGCACAGTCCGCCTTATCGCTTTGGTGAGGGGCATTTTTTCGTGGTGGGGTTTGGCCCGCGCTAGCCTCGTCTGGGTTCGGCAAAATGACCGCACTTGGTTTTTTTGCGACCGGCGTCTGGCCGACGCGCCTCCCCACTAGCGAAAAATGCTCTGACCACTCATGTACCGTCGTACGAGCAAAGGCTAACGCGCCCCACTACAGGAAGCCGCTGAGCTCGGCAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATCGACATTGCCCTCTGGAAGTTCGAGACCC |