Possible ID: Ampelomyces quisqualis
Classification: Powdery mildew
Phylum: Ascomycota
Class: Dothideomycetes
Order: Pleosporales
Family: Phaeosphaeriaceae
Isolation and culturing methods:
Isolated from soil located in the Bucknell University Greenhouse, on the 4th floor of the Biology building. Sample was placed on malt extract agar + chloramphenicol and allowed to grow for a week. Pure sample was obtained by sectioning a small sample of the growth onto MEA plate.
Culture appearance and growth:
Surface texture appears white and fuzzy 1wk into growth. Small black pores (>1mm in diameter) appear after 3wks of growth. Hyphae measure 4-5um wide, with septa present, but lacking clamp connections.
Spore production:
Cleistothecia are dark brown, measuring 62.5-67.5 um x 100-105um and develop on agar surface, not embedded. Spores are dikaryotic and oval shaped, measuring 2-3um.x 4-5um.
growth wk 1
growth week 5
cleistothecium
ETA: PCR sequencing and BLAST analysis suggest this speceies is Didymella americana, in the class Dothideomycetes.