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Issue: Vol. 630 No. 1: 12 Dec. 2023
Type: Article
Published: 2023-12-12
DOI: 10.11646/phytotaxa.630.1.3
Page range: 40-50
Abstract views: 76
PDF downloaded: 6

Eucalyptus leaf spot disease caused by Coniella eucalyptorum in Sri Lanka

Department of plant sciences,faculty of science, University of Colombo
Center for Yunnan Plateau Biological Resources Protection and Utilization; Qujing Normal University; Qujing; Yunnan Province; 655011 P.R. China. Tropical Microbiology Research Foundation; 96/N/10; Meemanagoda Road; 10230 Pannipitiya; Sri Lanka.
Department of Plant Sciences; Faculty of Science; University of Colombo; Colombo 00700; Sri Lanka.
State Timber Corporation Sri Lanka; No.82; “Sampath Paya”; Rajamalwatte Road; Sri Jayawardenepura Kotte; 10100 Sri Lanka.
National Institute of Fundamental Studies; Hanthana; Kandy; 20000; Sri Lanka.
Center for Yunnan Plateau Biological Resources Protection and Utilization; Qujing Normal University; Qujing; Yunnan Province; 655011 P.R. China.
Department of Plant Sciences; Faculty of Science; University of Colombo; Colombo 00700; Sri Lanka.
Foliar pathogen New geographical record Pilidella South Asia Timber trees Fungi

Abstract

Coniella has a wide distribution as a plant pathogen in Eucalyptus species. Here we report a leaf spot disease caused by Coniella eucalyptorum on Eucalyptus camaldulensis in Sri Lanka. The pathogen was isolated from symptomatic leaf samples of E. camaldulensis plants from Polonnaruwa District in Sri Lanka. The isolated fungus was identified as C. eucalyptorum based on morphological characteristics and multi-gene phylogenetic analysis of combined sequences of the internal transcribed spacer region (ITS), the partial translation elongation factor 1-α gene (tef1-α), and large subunit of nuclear ribosomal RNA (LSU). The pathogenicity test was performed to confirm its pathogenicity to the host, which produced symptoms similar to those observed in the field. This is the first report of C. eucalyptorum associated with Eucalyptus plantations in Sri Lanka. This finding is important to expand the knowledge of plant pathogen diversity in Eucalyptus species and other economically important crops in Sri Lanka.

 

References

  1. Alvarez, L.V., Groenewald, J.Z. & Crous, P.W. (2016) Revising the Schizoparmaceae: Coniella and its synonyms Pilidiella and Schizoparme. Studies in Mycology 85: 1–34. https://doi.org/10.1016/j.simyco.2016.09.001
  2. Amarasinghe, W.T.D., Terada, T. & Yamamoto, H. (2021) Enhancing the carbon sequestration of high-elevation Eucalyptus plantations in Sri Lanka for future carbon market activities. Journal of Forest Research 26: 351–357. https://doi.org/10.1080/13416979.2021.1927501
  3. Andjic, V., Carnegie, A.J., Pegg, G.S., Hardy, G.E.S.J., Maxwell, A., Crous, P.W., Pérez, C., Wingfield, M.J. & Burgess, T.I. (2019) 23 years of research on Teratosphaeria leaf blight of Eucalyptus. Forest Ecology and Management 443: 19–27. https://doi.org/10.1016/j.foreco.2019.04.013
  4. Bayle, G. (2019) Ecological and social impacts of Eucalyptus tree plantation on the environment. Journal of Biodiversity Conservation and Bioresource Management, 5: 93–104. https://doi.org/10.3329/jbcbm.v5i1.42189
  5. Bhunjun, C.S., Phillips, A.J., Jayawardena, R.S., Promputtha, I. & Hyde, K.D. (2021) Importance of molecular data to identify fungal plant pathogens and guidelines for pathogenicity testing based on Koch’s Postulates. Pathogens 10: 1096. https://doi.org/10.3390/pathogens10091096
  6. Carnegie, A.J. (2007) Forest health condition in New South Wales, Australia, 1996–2005. II. Fungal damage recorded in eucalypt plantations during forest health surveys and their management. Australasian Plant Pathology 36: 225–239. https://doi.org/10.1071/AP07021
  7. Chen, Y., Fu, D., Wang, W., Gleason, M.L., Zhang, R., Liang, X. & Sun, G. (2022) Diversity of Colletotrichum species causing apple bitter rot and Glomerella leaf spot in China. Journal of Fungi 8: 740. https://doi.org/10.3390/jof8070740
  8. Chen, Z.Z., Ho, C.K., Ahn, I.S. & Chiang, V.L. (2006) Eucalyptus, Agrobacterium Protocols. Methods in Molecular Biology 2: 344. https://doi.org/10.1385/1-59745-131-2:125
  9. Crous, PW (1998) Mycosphaerella spp. and their anamorphs associated with leaf spot diseases of Eucalyptus. Mycologia Memoir 21: 1–170. https://doi.org/10.2307/3761362
  10. Crous, P.W., Wingfield, M.J. & Burgess, T.I. (2016) Fungal Planet description sheets: 469–557. Persoonia 37: 218–403. https://doi.org/10.3767/003158516X694499
  11. Crous, P.W., Michael, J., Wingfield, R., Cheewangkoon, A.J., Carnegie, T.I., Burgess, B.A., Summerell, J., Edwards, Taylor, P.W.J. & Johannes, Z.G. (2019) Foliar pathogens of Eucalypts. Studies in Mycology 94: 125–298. https://doi.org/10.1016/j.simyco.2019.08.001
  12. Greyling, I., Wingfield, M.J., Coetzee, M.P., Marincowitz, S. & Roux, J. (2016) The Eucalyptus shoot and leaf pathogen Teratosphaeria destructans recorded in South Africa. Southern Forests: a Journal of Forest Science 78 (2): 123–129. https://doi.org/10.2989/20702620.2015.1136504
  13. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.1021/bk-1999-0734.ch008
  14. Hyde, K.D., de Silva, N.I., Jeewon, R., Bhat, D.J., Phookamsak, R.,Doilom, M., Boonmee, S., Jayawardena, R.S., Maharachchikumbura, S.S.N.,Senanayake, I.C., Manawasinghe, I.S., Liu, N., Abeywickrama, P.D., Chaiwan, N., Karunarathna, A., Pem, D., Lin, C.G., Sysouphanthong, P., Luo, Z.L., Wei, D.P., Wanasinghe, D.N., Norphanphoun, C., Tennakoon, D.S., Samarakoon, M.C., Jayasiri, S.C., Jiang, H.B., Zeng, X.Y., Li, J.F., Wijesinghe, S.N., Devadatha, B., Goonasekara, I.D., Brahmanage, R.S., Yang, E.F., Aluthmuhandiram, J.V.S., Dayarathne, M.C., Marasinghe, D.S., Li, W.J., Dissanayake, L.S., Dong, W., Huanraluek, N., Lumyong, S., Liu, J.K., Karunarathna, S.C., Jones, E.B.G., Al-Sadi, A.M.,Xu, J.C., Harishchandra, D., Sarma, V.V. & Bulgakov, T.S. (2020) AJOM new records and collections of fungi: 1–100. Asian Journal of Mycology 3: 22–294. https://doi.org/10.5943/ajom/3/1/3
  15. Index Fungorum (2022) Index Fungorum. Available from: http://www.indexfungorum.org/names/Names.asp (accessed: 10 June 2023).
  16. Jayawardena, R.S., Hyde, K.D., de Farias, A.R.G., Bhunjun, C.S., Ferdinandez, H.S., Manamgoda, D.S., Udayanga, D., Herath, I.S., Thambugala, K.M., Manawasinghe, I.S., Gajanayake, A.J. Samarakoon, B.C., Bundhun, D., Gomdola, D., Huanraluek, N., Sun, Y., Tang, X., Promputtha, I. & Thines, M. (2021) What is a species in fungal plant pathogens? Fungal Diversity 109: 239–266. https://doi.org/10.1007/s13225-021-00484-8
  17. Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  18. Nylander, J.A.A. (2004) MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, pp. 2–4.
  19. Park, R.F., Keane, P.J., Wingfield, M.J. & Crous, P.W. (2000) Fungal diseases of Eucalypt foliage. Diseases and pathogens of Eucalypts 153–239.
  20. Rambaut, A. (2012) Fig.Tree. v. 1.4.0. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed: 1 May 2023).
  21. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  22. Senanayake, I.C., Rathnayaka, A.R., Marasinghe, D.S., Calabon, M.S., Gentekaki, E., Lee, H.B., Hurdeal, V.G., Pem, D., Dissanayake, L.S., Wijesinghe, S.N., Bundhun, D., Nguyen, T.T., Goonasekara, I.D., Abeywickrama, P.D., Bhunjun, C.S., Jayawardena, R.S., Wanasinghe, D.N., Jeewon, R., Bhat, D.J. & Xiang, M.M. (2020) Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere 11: 2678–2754. https://doi.org/10.5943/mycosphere/11/1/20
  23. Shear, C.L. (1923) Life histories and undescribed genera and species of fungi. Mycologia 15: 120–131. https://doi.org/10.1080/00275514.1923.12020405
  24. Silvestro, D. & Michalak, I. (2012) raxmlGUI: a graphical front-end for RAxML. Organisms Diversity & Evolution 12: 335–337. https://doi.org/10.1007/s13127-011-0056-0
  25. Sivananthawerl, T. & Mitlöhner, R. (2011) Eucalyptus grandis and other important Eucalyptus species: A case study from Sri Lanka. Silviculture in the Tropics 463–472. https://doi.org/10.1007/978-3-642-19986-8_29
  26. Smith, A.H., Wardlaw, T.J., Pinkard, E.A., Ratkowsky, D. & Mohammed, C.L. (2017) Impacts of Teratosphaeria leaf disease on plantation Eucalyptus globulus productivity. Forest Pathology 47 (2): e12310. https://doi.org/10.1111/efp.12310
  27. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729. https://doi.org/10.1093/molbev/mst197
  28. Von Höhnel, F. (1918) Dritte vorlaufige Mitteilung mycologischer Ergebnisse (Nr.201–304). Berichte der Deutschen Botanischen Gesellschaft 36: 309–317.
  29. White, T.J., Bruns, T., Lee, S.J.W.T. & Taylor, J.L. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18: 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  30. Wijayawardene, N.N., Phillips, A.J.L., Pereira, D.S., Dai, D.Q., Aptroot, A., Monteiro, J.S., Druzhinina, I.S., Cai, F., Fan, X., Selbmann, L., Coleine, C., Castañeda-Ruiz, R.F., Kukwa, M., Flakus, A., Fiuza, P.O., Kirk, P.M., Kumar, K.C.R., Arachchi, I.S.l, Suwannarach, N., Tang, L.Z., Boekhout, T., Tan, C.S., Jayasinghe, R.P.P.K. & Thines, M. (2022a) Forecasting the number of species of asexually reproducing fungi (Ascomycota and Basidiomycota). Fungal Diversity 114 (1): 463–490. https://doi.org/10.1007/s13225-022-00500-5
  31. Wijayawardene, N.N., Rajakaruna, S, Dai, D.Q., Jayasekara, S., Warnakula, L., Ariyawansa, K.G.S.U., Fernando, E.Y., Jayasekera, P., Karunarathna, S.C., Singhalage, D., Ukuwela, K., Jayalal, R.G.U., Jayasinghe, R.P.P.K., Muthumala, C.K., Madawala, S., Hewajulige, I.G.N., Rajawardana, D.U., Ediriweera, A., Ediriweera, S., Alawathugoda, D., Rajawatta, K.M.W., Jin, X.C., Chandana, E.P.S., Nanayakkara, C. & Wijesundara, S. (2022b) Necessity of a National Fungarium and a Culture Collection for Fungi in Sri Lanka. Chiang Mai Journal of Science 49 (2): 248–271. https://doi.org/10.12982/CMJS.2022.027