3. Introduction
• Sporotrichum is a filamentous fungus widely
distributed in decaying wood and soil. It is
commonly considered as a contaminant
• Sporotrichum species comprise a very small
proportion of the fungal biota and are most
closely related to other colorless hyphomycetes
such as Chrysosporium
• Of several species in the genus, only
Sporotrichum schenckii, Sporotrichum
pruinosum is notable pathogen to man and
animals
5. • Sporotrichum differs from Chrysosporium
and Sporothrix by the typical clamp
connections and thick-walled, unicellular,
golden yellow conidia with annular frills
• It differs from Sporothrix also by not being
dimorphic. Sporotrichum differs from
Emmonsia parva by producing (adiaspore-
like) chlamydospores not only at 37°C
but also at 25°C
7. Mophology
• Dimorphic form- mold and yeast
• Shape - oval to elongate cigar shaped
approximately 2 miron by 4 micron
• Color is initially white and then becomes
rosy beige( pale sandy fawn color),
pinkish, yellow or orange
• Colonies usually become wrinkled, and the
central portions develop brown to black
pigmentation as they mature
• Mould form - slender, segmented
mycelium with pyriform conidia occuring in
rows mycelium and in terminal clusters
9. Growth
• Mold form grows fairly rapidly on selective
mediums containing cyclohexamide and
chlororamphenicol
• Yeast or tissue may be produced by persistent
culturing on fresh blood agar at 37 degree
celcius
• Grow rapidly and mature within 5-7 days at 25
degree celcius
• The texture is velvety to powdery.
• From the front, the color is initially white and
then becomes rosy beige( pale sandy fawn
color), pinkish, yellow or orange.
10. Pathogenesis
• The only information available regarding health
effects are a few rare cases of repeated
isolations from respiratory secretions suggestive
of bronchopulmonary colonization
• Colonization of the bronchopulmonary tree with
Sporotrichum pruinosum is likely. Also, the
organism was shown to produce giant, thick-
walled chlamydospore-like structures similar to
the adiaspores of Emmonsia parva in tissues
• No information is available regarding toxicity
• Allergencity has not been studied.
11. Diagnosis
1. Culturing/ isolation of fungus from swab or a biopsy of
the infected site
2. AGGLUTINATION
3. IMMUNODIFFUSION (ID)
4. COMPLEMENT FIXATION TEST (CFT)
5. ENZYME LINKED IMMUNOSORBENT ASSAY(ELISA)
• LATERAL FLOW ASSAY
6. COUNTER IMMUNO-ELECTROPHORESIS (CIE)
7. RADIO IMMUNOSORBENT ASSAY (RIA)
• CARBOHYDRATE FERMENTATION TEST:
Fermentation tests detect whether yeast can
produce gas from the carbohydrate under
anaerobic conditions
12. Fig. 1 Gram stain of fine needle aspirate of the
nodule (1000×) showing intracellular yeast cells
with multiple budding inside foamy and debris
laden macrophage.
