MOESM2 of Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants

Additional file 2. A list of Additional Figures and Tables. Figure S1. Library preparation flowchart of MiddRAD protocol A. Figure S2. In silico digestion genome sequences of 23 plant species by EcoRI + MspI and PstI + MspI. Figure S3. Fragments distribution of library A and library B. Figure S4. Maximum likelihood phylogenetic reconstruction of three bamboo species. Table S1. Species adopted for in silico digestion and the corresponding genome size. Table S2. Restriction enzymes included in this study. Table S3. Independent Sanger sequencing for genotype validation of MiddRAD-seq genotyping. Table S4. Comparison of most commonly used RAD and GBS sequencing methodologies and associated costs.