Ş. Kabaktepe, B. Mutlu, Ş. Karakuş / Hacettepe J. Biol. & Chem., 2013, 41 (3), 221-224 New records of Microfungi from Malatya province in Turkey Türkiye’de Malatya İlinden Yeni Mikrofungus Kayıtları Research Article Şanlı Kabaktepe*, Birol Mutlu, Şükrü Karakuş Department of Biology, Science and Art Faculty, İnönü University, Malatya, Turkey ABSTR AC T T hree microfungi taxa, Pleospora lithophilae Gucevič on Pimpinella paucidentata V.A.Matthews, Septoria cruciatae Roberge ex Desm. on Galium mite Boiss. & Hohen., Placosphaeria campanulae (DC.) Bäumler on Asyneuma amplexicaule Hand.–Mazz. var. angustifolium (Boiss.) Bornm., are reported for the first time from Turkey. The morphological and microscopical features with figures of these fungi are described based on the collected materials. Key words Malatya, Microfungi, Ascomycota, Turkey. ÖZET impinella paucidentata V.A.Matthews üzerinde Pleospora lithophilae Gucevič, Galium mite Boiss. & Hohen. üzerinde Septoria cruciatae Roberge ex Desm. ve Asyneuma amplexicaule Hand.–Mazz. var. angustifolium (Boiss.) Bornm. üzerinde Placosphaeria campanulae (DC.) Bäumler olan 3 tane mikrofungus türü Türkiye’den ilk defa kaydedilmiştir. Bu fungusların şekilleri ile morfolojik ve mikroskobik özellikleri toplanan örneklere bağlı olarak tanımlanmıştır. P Anahtar Sözcükler Malatya, Mikrofus, Ascomycota, Turkey. Article History: Received May 30, 2013; Revised June 27, 2013; Accepted July 20, 2013; Avaliable Online: September 1, 2013 Correspondence to: Şanlı Kabaktepe, Department of Biology, Science and Art Faculty, İnönü University, Malatya, Turkey Tel:+90 422 377 37 02 Fax: +90 422 341 00 37 E-mail: birol.mutlu@inonu.edu.tr 222 Ş. Kabaktepe, B. Mutlu, Ş. Karakuş / Hacettepe J. Biol. & Chem., 2013, 41 (3), 221-224 INTRODUCTION P lant diseases are caused mainly by fungi. They often suffer a deficiency of water and minerals which results in discoloration, wilting, desiccation and reduced growth. For effective control of a disease, firstly need to know the causal agent. The floristic studies of the microfungal flora are the first and the most important step to control the fungal diseases in a country. In the last decade, studies about microfungi fungi have greatly increased in Turkey. Nevertheless, numerous new records and new species can still be expected as result of on going fieldwork because of its high diversity of vascular plants (9996 species) in Turkey [1]. This paper presents rust species collected from Malatya province in Turkey. MATERIALS AND METHODS Materials were collected in 1996 and 2012 from Malatya Province in Turkey. The host specimens were prepared according to established herbarium techniques. Host plants identified use the “Flora of Turkey and the East Aegean Islands” [2-4]. Spores were scraped from dried host specimens and mounted in lactophenol. Analysis LS Starterwas software used to measure. The current names of fungi are given according to www.indexfungorum.org. Names of host plants and families are given according to http://www. ipni.org and http://www.theplanlist.org. Voucher specimens are deposited in the Inönü University Herbarium (INU). RESULTS AND DISCUSSION Ascomycota Whittaker Dothideomycetes O.E. Erikss. & Winka Pleosporales Luttr. ex M.E. Barr Pleosporaceae Nitschke Pleospora Rabenh. ex Ces. & De Not. Pleospora is widespread on plants, causing leaf spots with 63 species [5]. 24 species of Pleospora are now known from Turkey [6-10]. Pleospora lithophilae Gucevič (Figure 1A, D, G, H) Pseudothecia supepidermal up to 0.3 mm, black. Ascospores yellow to brown, 5–7 transversa and 1 longitudinal septa, 20–35 × 7–15 μm, rounded at the both base. Specimen examined: On Pimpinella paucidentata V.A.Matthews (Apiaceae). Turkey: B7 Malatya, Kozluk valley, limestone, east slopes, 1100–1200 m, 08.09.1996, B.Yıldız 14114 (INU 3156). Remarks: Known from Asia and Europe. P. lithophilae is a new microfungi species for Turkey. P. lithophilae is also the first Pleospora species on Pimpinella species in Turkey. Capnodiales Woron. Mycosphaerellaceae Lindau Septoria Sacc. Septoria is widespread on plants with 1072 species [5]. All the species are parasitic typically causing leaf spots. 82 species of Septoria are now known from Turkey [11-14]. Septoria cruciatae Roberge ex Desm. (Figure 1B, E, I) Spots amphigenous, ovoid, irregular, yellowish– brown. Picnidia epiphyllous, blackish brown, pierced by a pore. Spores elongate, curved, (18-) 25–50 × 1–2 μm. Specimen examined: On Galium mite Boiss. & Hohen. (Rubiaceae). Turkey: B7 Malatya, Arguvan, Kuruttaş village, Hasbek hill, 1700 m, 06.07.2012, Ş.Karakuş 2833 & B.Mutlu (INU 1198). Remarks: Known from Asia, Europe and North America. S. cruciatae is a new microfungi species for Turkey. S. cruciatae is also the first Septoria species on Galium species in Turkey. Dothideales Lindau Incertae sedis Placosphaeria (De Not.) Sacc. Placosphaeria is widespread on plants with 50 species [5]. 2 species of Placosphaeria are now known from Turkey [15, 16]. Ş. Kabaktepe, B. Mutlu, Ş. Karakuş / Hacettepe J. Biol. & Chem., 2013, 41 (3), 221-224 Figure 1. Herbarium specimens (A, B.Yıldız 14114; B, Ş.Karakuş 2833; C, Ş.Karakuş 2802), steromicroscope image of infected area on the leaves tissue (D–F) and lightmicroscope image of ascus (G), ascospores (H) and conidiospores (IJ) of Pimpinella paucidentata V.A.Matthews (A, D), Galium mite Boiss. & Hohen. (B, E), Asyneuma amplexicaule (Willd.) Hand.-Mazz. var. angustifolium (Boiss.) Bornm. (C, F), Pleospora lithoplilae Gucevič (G-H), Septoria cruciatae Roberge ex Desm. (I) and Plachosphaeria campanulae (DC.) Bäumler (J). 223 224 Ş. Kabaktepe, B. Mutlu, Ş. Karakuş / Hacettepe J. Biol. & Chem., 2013, 41 (3), 221-224 Placosphaeria campanulae (DC.) Bäumler (Figure 1C, F, J) Spots amphigenous, in clusters, blackish brown. Spores cylindrical, 2–5 × 1μm, hyaline. Specimen examined: On Asyneuma amplexicaule (Willd.) Hand.–Mazz. var. angustifolium (Boiss.) Bornm. (Campanulaceae). Turkey: B7 Malatya, Arapkir, Göldağı mountain, 1800 m, 04.07.2012, Ş.Karakuş 2802 & B.Mutlu (INU 1197). Remarks: Known from Asia and Europe. P. campanulae is a new microfungi species for Turkey. P. campanulae is also the first Placosphaeria species on Asyneuma species in Turkey. ACKNOWLEDGEMENTS The plant samples of collector named “Ş.Karakuş” were collected during Phd study of Şükrü Karakuş. This study was supported by XV Regional Directorate of the Ministry of Forestry and Water Affairs of the Republic of Turkey. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. REFERENCES 1. 2. A. Güner, S. Aslan, T. Ekim, M. Vural, M.T. Babaç, Türkiye Bitkileri Listesi (Damarlı Bitkiler). 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