日 植 病 報60: Ann. 608‑612 Phytopath. (1994) Soc. Brown 短 Japan 60: Zonate 608‑612 Spot Hideo (1994) of Grape NASU*, Akira and Key words: In was the autumn found fungus in and of V. easily. Many synnemata of Muscat in autumn sterilized on agar were of The needle. Discs of on The the 1976 and leaves The of cleaned Teleki 5BB Cav., grape, the were causal The cut fungal at length leaves I-2). grape lesions leaves were transferred cultures (isolates of Okayama of then fell (Plate Sanyo-cho, and leaves. 3-5cm surface diseased from were of diseased and conidia the Teleki distilled 5BB, water, cut from on injured with were and the infected leaves placed on from S-1, for potato lesions to 2 (IFO32598)) were of the on the a humid in experi- with a colony on on leaves areas only kept inoculation slightly uninjured discs leaves used injured periphery and fungal inoculated zonate leaf water, leaves portion The lower Hilohumburg grape experiments. and inoculated parts. and clear. on pieces muscat, of marginal Tsuyama-shi with placed the shape distilled and diameter Neo Naturally in inoculation with individually scorched-needle-wounded ampelophaga of spots was lesion fields lesions, Muscat in in area sterilized and Neo 5mm were at the synnemata on studies grape, developed Tissue with conidia were Briosia disease cultivar zonate experiments. the respectively. dishes. with L. healthy in in washed of conidia leaves 1993, petri by new 5BB), non-circular the produced collected approximately with were morphological of and inoculation varieties surface synnemata Muscat. for two mostly lesion and 9cm mostly color, were synnemata used Leaves ments. in The conidia ethanol, caused the vinifera Teleki lesions 5BB 70% (PSA) PSA. PSA of with in the fungus Teleki grape, describes (Vitis variety, The brown with the and Prefecture, of to of report vineyards 1993. between ITO* ampelophaga. disease This rootstock and ampelophaga Japan. In 1976 boundary Isolation sucrose of Tadayoshi Note HATAMOTO* unknown in disease. brown The an by Briosia NAKAGIRI**, Briosia Japan. riparia reddish I-1). slants the autumn were 1993, experiments of in were and Caused Motomu spot, grape, Prefecture, inoculation (Plate Neo 1976 berlandieri•~V. observed They zonate Okayama Symptom and brown 報 Phytopathological sterilized PSA and of Neo needle-wounded box at and 25•Ž for 6 days. Two Muscat days and synnemata on lower Zonate on a brown after the were of the which a light of spot leaves appeared to (Plate on No leaves on the lesions those I-3). infected observed on inoculation, similar fungus were the naturally and 700•~50-250(400•~150 and ＊Okayama 験 場 ＊＊Institute on conidia. (Plate Prefectural Fermentation in a scanning composed the wound-inoculated expanded up naturally symptoms in made the 3cm on with field, did in leaves observed wounds leaves leaves to infected were through infected at the Station Osaka branched, 532, was apices were with produced (Hitachi synnema cells Experiment I-4) were microscope Each produced , Yodogawa‑ku, and interwoven, Conidiogenous Agricultural (Plate field loosely diam. were I-5). synnemata the electron of average)ƒÊm Conidiophores hyaline for The leaves and hyaline, of fungus. diseased microscope hypophyllous, septate, which that spots, Morphology a mass days were Neo appear and observed uninjured a needle not of diameter, leaves. or a scorched on artificially leaves. observed under Four surface suggested inoculated inoculation, conidia the was needle. the 5BB. with mainly It after Teleki thallic, , Sanyo‑cho, Japan財 of conidia by which S-800). septate, capped synnemata, by examined were and hyphae, hyaline a brown head macronematous, integrated, 709‑08, 団 法 人 発 酵 研 究 所 sometimes were Synnemata meristematic, Okayama were inoculation, Japan岡 mostly composed 300of branched, terminal and 山 県 立 農 業 試 Ann. Phytopath. cylindrical. Conidia branched were basipetal more particularly so non-septate, Colonies of of temperatures at synnemata on on Optimum on PCA PSA. The within up to of in This dating on has on the Lindau the as species was 25•Ž. The the for its cubical Alberta Press, J. M.B. L. Sutton, 132: 和 文 PSA brown, at conidia various a few of Sclerotia few II-4), temperatures in size synnemata but none than agar II-2) apices 2-5mm A (Plate the days. masses sclerotia. the (Plate at after hyphal occupied 35•Ž surface leaves to pale brown, from of Mason3). very in were on 9cm PSA. petri off Europe, Further and North is the spherical of B. chains has double Brazil. To (Peck) conidia recognized on or a azaleae of been often catenate, and as studies Briosia threads America acropetal species 80-400•~ were splitting spp. taxonomic Thus, with the with were becoming by Rhododendron the Stilbellaceae Conidia secede forming Fries1). the synnemata They ampelophaga, Sorocybe in The rounding size. conidiogenesis (Peck) (1888)3-5) bellow3). meristematic. but in fungus B. Cavara thallic, Vitis parasitic in genus zonate its spot of pathogenicity the new name disease of grape to of common new J.W., Kendrick, Canada. (1941). (1976). and arthroconidia. 5. fungus by of leaves leaves of zonate brown which to belong Pycnostysanus monotypic genus may accommo- B.S. New More spot of and grape spot Japan coincides with Briosia morphology. in Japan (•gKappan was identified hagarebyo•h in as Briosia Japanese) is Conners, I.L. cited and Sigler, L. (1980). Genera of Hyphomycetes. The Univ. 157. species of Tennessee Dematiaceous Carmichael, J.W. Mycotaxon. 4: (1973). Okayama, grape. W.B., p. in grape zonate Literature Sigler, of higher hyaline or blastic azaleae the fungus The this Carmichael, 4. size leaves at reported were from a formation P. was 5-10ƒÊm reported from to causal Cav. Ellis, Many produced produced infected described cells oblong of to isolated respect Therefore, Dearness, to ampelophaga. in 3. in and Temperature on those in consisted grow macronematous, assigned conidium thallic fungus 2. formed during brown observed. rates white. to and to verruculose, been because ampelophaga of pale were grayish similar and failed fungus were to However, ampelophaga 1. angular, growth average)ƒÊm black, was It's initially the Briosia proposed was transparent, those Conidiogenous Dearness2) transfer The PSA and Briosia smooth fungus3-5) Pycnostysanus to apex. Briosia, from lead fungus. non-septate, Dearrness4). to and off size. (PCA) mycelial were grey to 10•Ž, growth Conidiophores brown, genus to to at ampelophaga. the subspherical, differs the size. near schizogenous, the on (7•~5 white similar grew mycelial Briosia 500ƒÊm septum. (S-1) black were agar the 5-13•~3-8 thin, to in I-6) rounded days. isolate and cubical carrot (Plate become average)ƒÊm conidiophores was and for species, branched the but 7 days. Taxonomy a single of on 609 succession cubical spherical, potato 6 basipetal to determining for smooth, PCA fungus temperature dishes days, on and mononematous lesions, 20 Mycelia formed observed II-1) of infected after II-3). were top the produced (Plate (Plate the a septum, by 35•Ž in (6.8•~6.2 PSA studied 5 to down oblong 5-10•~4-8 on was from colony chains were isolates laid initially by I-7), fungus ranging Mycelial with the septa were delimitation (Plate the by They after rugulose responses delimited chains. Soc. Japan 60 (5). October, 1994 fungi. Mycologia Hyphomycetes. (1976). Taxonomy 33: C.M.I., of Kew, Malbranchea 360-366. England. and some pp. 24-25, other 154-156. Hyphomycetes with 372-379. Hyphomycetes from Manitoba and Saskatchewan, Canada. Mycological Papers C.M.I. 84-86. 摘 要 那 須 英 夫 ・中 桐 昭 ・伊 藤 忠 義 ・畑 本 岡 山 県 に お い て,1976, 1993年 の 秋,ブ 求:Briosia ampelophagaと よ る ブ ド ウ褐 斑 葉 枯 病 ド ウ の 葉 に 未 報 告 の 葉 枯 性 病 害 が 発 生 し た 。本 病 は 葉 に 発 生 し,病 縁 か ら生 じ て 褐 変 し,次 第 に 拡 大 し て3〜5cmの 性 か ら病 原 菌 をBriosia ampelophagaに 斑 は葉 大 き な 不 定 形 の 病 斑 と な る 。形 態 的 な 特 徴 及 び ブ ド ウ に 対 す る病 原 同 定 し,病 名 と し て 褐 斑 葉 枯 病(brown zonate spot)を 提 唱 した。 本菌 は 610 日本植 物 病 理 学会 報 第60巻 第5号 平成6年10月 本 邦 未 記載 で あ る。 (Received April 25, 1994) Explanation Plate of plates I 1. Lesion 2. Synnemata of naturally of 3. Synnemata produced ampelophaga leaves of ampelophaga in a grape (Vitis produced lesion on on the lower vin(era the L. lower cv. surface surface of grape Neo Muscat). of a lesion. leaves Magnification 5. The 6. Conidia 7. Scanning of apices of and synnemata on a synnema of conidiophores electron an infested Briosia of micrograph Briosia of leaf. ampelophaga Colony 2. Conidia 3. Sclerotia 4. Synnemata inoculation (S-1). ampelophaga conidia Bar=500ƒÊm. obtained Bar=10ƒÊm. (S-1). Bar=10ƒÊm. from air-dried specimens. II 1. by (S-1). 4. Plate infested Briosia of Briosia produced formed produced ampelophaga on on (S-1) on mononematous PSA on (25•Ž PCA PSA (25•Ž conidiophores for (20 20 days). days). Bar=500ƒÊm. for on 4 days). PSA. Bar=20ƒÊm. Bar=5ƒÊm. with Briosia Ann. Phytopath. Plate I Soc. Japan 60 (5). October, 1994 611 612 Plate 日本 植 物 病 理 学会 報 II 第60巻 第5号 平 成6年10月