Proteinase K
- CAS No.
- 39450-01-6
- Chemical Name:
- Proteinase K
- Synonyms
- Protease K;EC 3.4.21.14;Tritirachium album proteinase K;20mg;bum;PROK;40U/MG;CHLOROM;C:C&B53;abs9265
- CBNumber:
- CB1399741
- Molecular Formula:
- C29H27N2O12P
- Molecular Weight:
- 626.504641
- MDL Number:
- MFCD00132129
- MOL File:
- 39450-01-6.mol
- MSDS File:
- SDS
| storage temp. | 2-8°C |
|---|---|
| solubility | H2O: 10 mg/mL, slightly hazy, brown-yellow |
| form | powder |
| color | white |
| PH | pH7.5～12 |
| Water Solubility | Miscible with water. |
| InChIKey | CDWGKFQEIBBJOF-BIZWTRPUNA-N |
| SMILES | N12[C@](OP(O)(OC3=CC=C([N+]([O-])=O)C=C3)=O)([C@H]([C@H](O)C)C1=O)[C@](C)(OC1=CC=CC=C1)C(OC1=CC=CC=C1)=C2C(OC)=O |&1:1,16,17,22,r| |
| CAS DataBase Reference | 39450-01-6 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H319-H334 |
| Precautionary statements | P261-P264-P280-P284-P302+P352-P305+P351+P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 23-24-26-36/37-22-45 |
| WGK Germany | 1 |
| F | 10 |
| HS Code | 35079090 |
Proteinase K price More Price(51)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 70663 | Proteinase K, Lyophilized Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. | 39450-01-6 | 100mg | $155 | 2024-03-01 | Buy |
| Sigma-Aldrich | 70663 | Proteinase K, Lyophilized Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. | 39450-01-6 | 500mg | $506 | 2024-03-01 | Buy |
| Sigma-Aldrich | 539480 | Proteinase K from Tritirachium album | 39450-01-6 | 25MG | $58 | 2024-03-01 | Buy |
| Sigma-Aldrich | 539480 | Proteinase K from Tritirachium album | 39450-01-6 | 100MG | $132 | 2024-03-01 | Buy |
| Alfa Aesar | J62051 | Proteinase K, Tritirachium album limber | 39450-01-6 | 100mg | $109.2 | 2021-12-16 | Buy |
Proteinase K Chemical Properties,Uses,Production
Description
Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].
Chemical Properties
Powder beige
Uses
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Uses
A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling.
Definition
Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.
General Description
Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains.
Biochem/physiol Actions
Proteinase K catalyzes the hydrolysis of keratin.
References
[1] Farrell, R. “Creating a Ribonuclease-Free Environment.”RNA Methodologies (Sixth Edition) 2023: 51-70.
Proteinase K Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Henan Alfa Chemical Co., Ltd | +8618339805032 | alfa4@alfachem.cn | China | 12755 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49390 | 58 |
| Jiangsu Boquan Biotechnology Co., Ltd. | +86-18168774353 | boquanshengwu003@boquansw.com | China | 248 | 58 |
| Xi’an Sengmei Biotech Co.,Ltd. | +8615398038360 | lionel@accenturebio.com | China | 288 | 58 |
| XI AN SGONEK BIOLOGICAL TECHNOLOGY CO., LTD | 15202951039 | jason@sgonekbio.com | China | 268 | 58 |
| LEAPCHEM CO., LTD. | +86-852-30606658 | market18@leapchem.com | China | 43348 | 58 |
| Hebei Mojin Biotechnology Co., Ltd | +8613288715578 | sales@hbmojin.com | China | 12456 | 58 |
| Hebei Yanxi Chemical Co., Ltd. | +8617531190177 | peter@yan-xi.com | China | 5993 | 58 |
| Anhui Ruihan Technology Co., Ltd | +8617756083858 | daisy@anhuiruihan.com | China | 994 | 58 |
| Hebei Jingbo New Material Technology Co., Ltd | +8619931165850 | hbjbtech@163.com | China | 1000 | 58 |
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View Lastest Price from Proteinase K manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-04-23 | Proteinase K
39450-01-6
|
US $6.00 / KG | 1KG | More than 99% | 2000KG/Month | Hebei Saisier Technology Co., LTD | |
 |
2024-04-18 | Proteinase K
39450-01-6
|
US $2.00 / kg | 1kg | 99.8% | 10000ton | Ouhuang Engineering Materials (Hubei) Co., Ltd | |
 |
2024-03-29 | Proteinase K
39450-01-6
|
US $6.00-0.20 / KG | 1KG | 99% | g-kg-tons, free sample is available | Henan Fengda Chemical Co., Ltd |
-
- Proteinase K
39450-01-6
- US $6.00 / KG
- More than 99%
- Hebei Saisier Technology Co., LTD
-
- Proteinase K
39450-01-6
- US $2.00 / kg
- 99.8%
- Ouhuang Engineering Materials (Hubei) Co., Ltd
-
- Proteinase K
39450-01-6
- US $6.00-0.20 / KG
- 99%
- Henan Fengda Chemical Co., Ltd
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