📃Scientific paper: Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses Abstract: Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia ( Hymenochaetales ) were validated. Many morphological characters of Fuscoporia overlap among the species, emphasizing the role of molecular identification for accuracy. The identities of 658 Fuscoporia GenBank internal transcribed spacer (ITS) sequences were assessed using ITS phylogeny, revealing 109 (16.6%) misidentified and 196 (29.8%) unspecified sequences. They were validated and re-identified based on the research articles they were published in and, if unpublished, based on sequences from the type, type locality-derived sequences, or otherwise reliable sequences. To enhance the resolution of species delimitation, a phylogenetic assessment of a multi-marker dataset (ITS + nrLSU + rpb2 + tef1 ) was conducted. The multi-marker phylogeny resolved five of the twelve species complexes found in the ITS phylogeny and uncovered five new Fuscoporia species: F. dolichoseta , F. gilvoides , F. koreana , F. reticulata , and F. semicephala . The validated ITS sequences in this study may prevent further accumulation of misidentified sequences in public databases and contribute to a more accurate taxonomic evaluation of Fuscoporia species. Discover the rest of the scientific article on es/iode ➡️https://etcse.fr/xKdeL
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📃Scientific paper: Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses Abstract: Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia ( Hymenochaetales ) were validated. Many morphological characters of Fuscoporia overlap among the species, emphasizing the role of molecular identification for accuracy. The identities of 658 Fuscoporia GenBank internal transcribed spacer (ITS) sequences were assessed using ITS phylogeny, revealing 109 (16.6%) misidentified and 196 (29.8%) unspecified sequences. They were validated and re-identified based on the research articles they were published in and, if unpublished, based on sequences from the type, type locality-derived sequences, or otherwise reliable sequences. To enhance the resolution of species delimitation, a phylogenetic assessment of a multi-marker dataset (ITS + nrLSU + rpb2 + tef1 ) was conducted. The multi-marker phylogeny resolved five of the twelve species complexes found in the ITS phylogeny and uncovered five new Fuscoporia species: F. dolichoseta , F. gilvoides , F. koreana , F. reticulata , and F. semicephala . The validated ITS sequences in this study may prevent further accumulation of misidentified sequences in public databases and contribute to a more accurate taxonomic evaluation of Fuscoporia species. Discover the rest of the scientific article on es/iode ➡️https://etcse.fr/xKdeL
Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses
ethicseido.com
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📃Scientific paper: Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses Abstract: Although there is a continuous increase in available molecular data, not all sequence identities in public databases are always properly verified and managed. Here, the sequences available in GenBank for Fuscoporia ( Hymenochaetales ) were validated. Many morphological characters of Fuscoporia overlap among the species, emphasizing the role of molecular identification for accuracy. The identities of 658 Fuscoporia GenBank internal transcribed spacer (ITS) sequences were assessed using ITS phylogeny, revealing 109 (16.6%) misidentified and 196 (29.8%) unspecified sequences. They were validated and re-identified based on the research articles they were published in and, if unpublished, based on sequences from the type, type locality-derived sequences, or otherwise reliable sequences. To enhance the resolution of species delimitation, a phylogenetic assessment of a multi-marker dataset (ITS + nrLSU + rpb2 + tef1 ) was conducted. The multi-marker phylogeny resolved five of the twelve species complexes found in the ITS phylogeny and uncovered five new Fuscoporia species: F. dolichoseta , F. gilvoides , F. koreana , F. reticulata , and F. semicephala . The validated ITS sequences in this study may prevent further accumulation of misidentified sequences in public databases and contribute to a more accurate taxonomic evaluation of Fuscoporia species. Continued on ES/IODE ➡️ https://etcse.fr/xKdeL ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.
Validation of Fuscoporia (Hymenochaetales, Basidiomycota) ITS sequences and five new species based on multi-marker phylogenetic and morphological analyses
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Project management | Ecology | Marine Biology | Molecular techniques and DNA | Fieldwork | Communication and teaching
🐺Ready for another story about wolves? This time it’s about the Korean Peninsula wolf! 🐺🦴🦷🧪🧬 At present, wolves are considered extirpated from the entire Korean Peninsula according to the Red Data Book of South Korea. By using molecular tools, we found that Korean wolves are closely related to East Chinese wolf populations and do not seem to be highly genetically differentiated and were not geographically isolated from the rest of the continental populations. Thus, our preliminary results suggest that the best option for reintroducing wolves to the Korean Peninsula would be with wolves from neighbouring regions, such as northern China. 🌏🐾 Read all about our study and findings here: https://lnkd.in/ecBqekfv #KoreanWolf #Genetics #Conservation
Conservation implications of elucidating the Korean wolf taxonomic ambiguity through whole‐genome sequencing
onlinelibrary.wiley.com
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Looking for a #PhD position? An interesting offer at Faculty of Science, Palacký University Olomouc – #evolution and adaptation of #cyanobacteria Read more: https://lnkd.in/giZjkfD8 #PhDjobs #PhDinCzechia #biology "(...) Cyanobacteria belong to the most important primary producers, thriving in all environments with sufficient light intensity for billions of years. They inhabit extreme environments such as deserts, therefore, they are adapted to broad temperature fluctuation, high UV radiation, and drought stress. With climate change, the drylands are expanding, and the importance of cyanobacteria rise. The PhD project will be focused on the transcriptome and epigenome response to environmental stresses (e.g. drought stress) in several species of cyanobacteria. The transcriptome of the strains will be sequenced by short reads using Illumina. Furthermore, the PhD student will use third-generation sequencing (Oxford Nanopore) to reconstruct epigenomic profiles in several related species of cyanobacteria. The epigenomic profiles will be investigated at the population and species levels. The expression profiles will be connected with phenotype, epigenome, and genome diversity. Altogether, the result will provide unique data for our understanding of the role of adaptation, selection, and environment in the speciation of cyanobacteria. (...)"
PhD position – evolution and adaptation of cyanobacteria
https://www.researchjobs.cz
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📃Scientific paper: Rearranging the Bird’s Nest Fungi: molecular review of internal clades in Cyathus (Nidulariaceae, Basidiomycota) Abstract: The genus Cyathus was established in 1768, but more in-depth taxonomic studies with the group only occurred after 1844. In the following years, changes in the infrageneric classification of Cyathus were proposed based mainly on morphology. With advances in phylogenetic studies, the morphological classifications were tested and a new subdivision into three groups was proposed in 2007. Based on the last two classifications, this work aims to expand and understand the internal phylogenetic relationships among the fungi of the genus Cyathus and examine how these relationships are reflected in the taxonomic classification, through molecular analyses covering most of the species in the group, based on materials obtained from type specimens deposited in major fungal collections worldwide, besides expanding sampling with tropical species. Molecular analyses followed the protocols available in the literature, including the design of specific primers for Cyathus . In the phylogenetic analysis, using Maximum Parsimony and Bayesian methods, sequences of ITS and LSU regions from 41 samples of 39 species of Cyathus , 26 were placed with some nomenclatural types. The monophyly of Cyathus was confirmed with maximum support in both tests, and the infrageneric groups of the most recent classification were unchanged, but the clade striatum showed segregation into four groups and three subgroups. The phylogenetic organization is supported morphological characters, and diagnoses are pres... Discover the rest of the scientific article on es/iode ➡️https://etcse.fr/ec
Rearranging the Bird’s Nest Fungi: molecular review of internal clades in Cyathus (Nidulariaceae, Basidiomycota)
ethicseido.com
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