CREG Antibody (30R): sc-100695

CREG Antibody (30R) is an IgG₁ κ mouse monoclonal CREG antibody (also designated CREG antibody) that detects the CREG protein of human origin by WB, IP, IF, IHC(P) and ELISA. CREG Antibody (30R) is available as the non-conjugated anti-CREG antibody. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. CREG (cellular repressor of E1A-stimulated genes) is a cellular protein that antagonizes transcriptional activation and cellular transformation by E1A. CREG was initially isolated in a yeast two-hybrid screen due to its interaction with the TATA-binding protein, TBP. Binding sites for E2F, a key transcriptional reg-ulator of cell cycle progression, are required for repression of the adeno-virus E2 promoter by CREG, and CREG was shown to inhibit activation by E2F. CREG is broadly expressed in adult tissues and is regulated during embryonic development. CREG is a secreted glycoprotein which enhances differentiation of mouse embryonic stem cells and human NTERA-2 cells. CREG activity may contribute to the transcriptional control of cell growth and differentiation.

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References:

1. The secreted glycoprotein CREG enhances differentiation of NTERA-2 human embryonal carcinoma cells.  |  Veal, E., et al. 2000. Oncogene. 19: 2120-8. PMID: 10815803
2. Analysis of E1A-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity.  |  Stein, RW., et al. 1990. J Virol. 64: 4421-7. PMID: 2143544
3. Cellular targets for transformation by the adenovirus E1A proteins.  |  Whyte, P., et al. 1989. Cell. 56: 67-75. PMID: 2521301
4. Mechanism of active transcriptional repression by the retinoblastoma protein.  |  Weintraub, SJ., et al. 1995. Nature. 375: 812-5. PMID: 7596417
5. A cellular repressor of E1A-stimulated genes that inhibits activation by E2F.  |  Veal, E., et al. 1998. Mol Cell Biol. 18: 5032-41. PMID: 9710587

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CREG Antibody (30R) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CREG Antibody (30R) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CREG Antibody (30R) Specifications

Alternate Product Name

CREG

Product Clone Name

30R

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

121-221

Epitope Target

CREG

Epitope Species

human

Product Reactivity/Detection

CREG

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

2 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

N/A