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ERAP1 Antibody (B-10): sc-271823
- ERAP1 Antibody (B-10) is a mouse monoclonal IgG2a κ ERAP1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 721-840 mapping near the C-terminus of ERAP1 of human origin
- ERAP1 Antibody (B-10) is recommended for detection of ERAP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ERAP1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERAP1 (B-10): sc-271823.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for ERAP1 Antibody (B-10) for WB and IHC(P) applications. These reagents are now offered in bundles with ERAP1 Antibody (B-10) (see ordering information below).
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ERAP1 Antibody (B-10) is an IgG2a κ mouse monoclonal ERAP1 antibody (also designated ERAP1 antibody) that detects the ERAP1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. ERAP1 Antibody (B-10) is available as both the non-conjugated anti-ERAP1 antibody form, as well as multiple conjugated forms of anti-ERAP1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1) is localized to the lumen of the ER, which removes NH2-terminal residues from many antigenic precursors for MHC class I peptide presentation. ERAP1 is also designated adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1). Peptides presented by MHC class I on the surface of a cell must be eight to eleven residues long and ERAP1 specifically trims peptides of nine amino acids or more. ERAP1 is induced by interferon-g and encoded for by the ARTS1 gene, which maps to human chromosome 5q15. ERAP1 is thought to inactivate several bioactive peptides, including angioten-sin II, and subsequently, may be involved in the regulation of blood pressure. It may have a role in angiogenesis by regulating the proliferation and migration of endothelial cells, and is characterized as a TNFR1 binding protein that promotes TNFR1 shedding.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Characterization of recombinant human adipocyte-derived leucine aminopeptidase expressed in Chinese hamster ovary cells. | Hattori, A., et al. 2000. J Biochem. 128: 755-62. PMID: 11056387
- Genomic organization of the human adipocyte-derived leucine aminopeptidase gene and its relationship to the placental leucine aminopeptidase/oxytocinase gene. | Hattori, A., et al. 2001. J Biochem. 130: 235-41. PMID: 11481040
- Identification of 33 polymorphisms in the adipocyte-derived leucine aminopeptidase (ALAP) gene and possible association with hypertension. | Yamamoto, N., et al. 2002. Hum Mutat. 19: 251-7. PMID: 11857741
- Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding. | Cui, X., et al. 2002. J Clin Invest. 110: 515-26. PMID: 12189246
- Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is involved in the activation of endothelial integrins. | Akada, T., et al. 2002. J Cell Physiol. 193: 253-62. PMID: 12385003
- An IFN-gamma-induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides. | Saric, T., et al. 2002. Nat Immunol. 3: 1169-76. PMID: 12436109
- The ER aminopeptidase ERAP1 enhances or limits antigen presentation by trimming epitopes to 8-9 residues. | York, IA., et al. 2002. Nat Immunol. 3: 1177-84. PMID: 12436110
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERAP1 Antibody (B-10) | sc-271823 | 200 µg/ml | $310.00 | |||
ERAP1 Antibody (B-10): m-IgG Fc BP-HRP Bundle | sc-525826 | 200 µg Ab; 10 µg BP | $348.00 | |||
ERAP1 Antibody (B-10): m-IgG2a BP-HRP Bundle | sc-546431 | 200 µg Ab; 10 µg BP | $348.00 | |||
ERAP1 Antibody (B-10) AC | sc-271823 AC | 500 µg/ml, 25% agarose | $408.00 | |||
ERAP1 Antibody (B-10) HRP | sc-271823 HRP | 200 µg/ml | $310.00 | |||
ERAP1 Antibody (B-10) FITC | sc-271823 FITC | 200 µg/ml | $324.00 | |||
ERAP1 Antibody (B-10) PE | sc-271823 PE | 200 µg/ml | $337.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 488 | sc-271823 AF488 | 200 µg/ml | $350.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 546 | sc-271823 AF546 | 200 µg/ml | $350.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 594 | sc-271823 AF594 | 200 µg/ml | $350.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 647 | sc-271823 AF647 | 200 µg/ml | $350.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 680 | sc-271823 AF680 | 200 µg/ml | $350.00 | |||
ERAP1 Antibody (B-10) Alexa Fluor® 790 | sc-271823 AF790 | 200 µg/ml | $350.00 |