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In vitro degradation of plastic
- 1. IN VITRO DEGRADATION OF PLASTIC VIA MICROCOCCUS LUTEUS AND MASONIELLA SPECIES • Presented by – GAURAV GANGWAR
- 2. INTRODUCTION • WHAT IS PLASTIC? - A polymer material which become mobile on heating and thus can be cast into moulds. -durable and slowly degradable. • Adventage and Disadvantages
- 3. MATERIAL AND METHOD • COLLECTION OF SAMPLE 1. POLYSTYRENE COLLECTION. Collection of plastic cups from waste disposal. 2. SOIL COLLECTION. Any dumped area with large amount of plastic waste disposal.
- 4. METHODOLOGY • INOCULUM PREPARATION - NUTRIENT BROTH - POTATO DEXTROSE BROTH
- 5. PROCESSING OF SOIL SAMPLE • SERIAL DILUTION OF SOIL • INOCULATION OF 10-5 AND 10-6 DILUTION – BACTERIA ON NUTRITENT BROTH • 10-2 AND 10-3 – FOR FUNGI ON PDA
- 6. INDENTIFICATION • FOR BACTERIA *GRAM STAINING *MOTILITY TEST (HANGING DROP METHOD) *BIOCHEMICAL TEST – 1.IMViC TEST 2. CATALASE TEST 3. OXIDASE TEST • FOR FUNGI * LECTOPHENOL COTTON BLUE STAINING
- 7. MICROBIAL DEGRADATION OF PLASTIC • Surface Sterilization of Polystyrene. • Media Preparation. - 250ml of cooled Nutrient broth and potato dextrose broth was poured into four 500ml conical flasks. - The sterile pre-weighed plastic pieces were aseptically transformed into respective medium. - A loopful of Micrococcus Luteus a Masoniella was inoculated into respective medium. - These flasks were incubated at 37OC for 35, 45, and 55 days in shaking incubators.
- 8. RECOVERY • After 35 day of incubation, the removed pieces from culture were shade diced and weighed for final weight. The data were recorded. • The same procedure was also repeated for 45 and 55 days of incubation.
- 9. Determination of Degradation of Plastics • Measuring the weight lost by plastic after 35 of incubation. % weight loss = Initial Weight – Final Weight Initial weight x 100
- 10. RESULTS Isolation and identification of Micrococcus Luteus and Masoniella sp.
- 12. Degradation of plastics by Micrococcus Luteus:
- 13. Degradation of plastics by Masoniella sp
- 14. Biodegradation • The degradation of plastics by M.Luteus exposed at 35 days of incubation. Showed 19.6% of weight loss. Similarity degradation of 45 and 55 days of incubation showed 27.6%and 32 % respectively. • The degradation of plastics by Masoniella sp exposed at 35 days of incubation showed 8.8% of weight loss. Similarly degradation of 45 and 55 days of incubation showed 17.8% and 27.4% respectively.
- 15. Biodegradation of plastics by using Masoniella sp
- 16. Biodegradation of plastics by Micrococcus luteus
- 17. CONCLUSION • Micrococcus luteus and monosiella are very promising in degrading the plastic. • The bacteria caused the biodegration ranging from 19.6% to 32% for plastics among the bacteria Micrococcus luteus were found most active in degrade 38% of plastics. • The fungi caused the biodegradation ranging from 8.8% to 27.4% for plastics. Among the fungi Masoniella sp were found most active in degrading 27.4% of plastics.
- 18. THANK YOU