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Sporotrichum
- 1. SPOROTRICHUM Presented by: Pradeep singh Semester: 5th B.V.Sc and A.H. HICAST
- 2. Taxonomic Classification • Kingdom: Fungi • Phylum: Basidiomycota • Class: Hymenomycetes • Order: Stereales • Family: Corticiaceae • Genus: Sporotrichum • Species:Sporotrichum schenckii, Sporotrichum pruinosum
- 3. Introduction • Sporotrichum is a filamentous fungus widely distributed in decaying wood and soil. It is commonly considered as a contaminant • Sporotrichum species comprise a very small proportion of the fungal biota and are most closely related to other colorless hyphomycetes such as Chrysosporium • Of several species in the genus, only Sporotrichum schenckii, Sporotrichum pruinosum is notable pathogen to man and animals
- 4. Fig.:Chrysosporium( fig. for clearing doubt between Sporotrichum and Chrysosporium)
- 5. • Sporotrichum differs from Chrysosporium and Sporothrix by the typical clamp connections and thick-walled, unicellular, golden yellow conidia with annular frills • It differs from Sporothrix also by not being dimorphic. Sporotrichum differs from Emmonsia parva by producing (adiaspore- like) chlamydospores not only at 37°C but also at 25°C
- 6. Fig.: Multiplication of fungi
- 7. Mophology • Dimorphic form- mold and yeast • Shape - oval to elongate cigar shaped approximately 2 miron by 4 micron • Color is initially white and then becomes rosy beige( pale sandy fawn color), pinkish, yellow or orange • Colonies usually become wrinkled, and the central portions develop brown to black pigmentation as they mature • Mould form - slender, segmented mycelium with pyriform conidia occuring in rows mycelium and in terminal clusters
- 9. Growth • Mold form grows fairly rapidly on selective mediums containing cyclohexamide and chlororamphenicol • Yeast or tissue may be produced by persistent culturing on fresh blood agar at 37 degree celcius • Grow rapidly and mature within 5-7 days at 25 degree celcius • The texture is velvety to powdery. • From the front, the color is initially white and then becomes rosy beige( pale sandy fawn color), pinkish, yellow or orange.
- 10. Pathogenesis • The only information available regarding health effects are a few rare cases of repeated isolations from respiratory secretions suggestive of bronchopulmonary colonization • Colonization of the bronchopulmonary tree with Sporotrichum pruinosum is likely. Also, the organism was shown to produce giant, thick- walled chlamydospore-like structures similar to the adiaspores of Emmonsia parva in tissues • No information is available regarding toxicity • Allergencity has not been studied.
- 11. Diagnosis 1. Culturing/ isolation of fungus from swab or a biopsy of the infected site 2. AGGLUTINATION 3. IMMUNODIFFUSION (ID) 4. COMPLEMENT FIXATION TEST (CFT) 5. ENZYME LINKED IMMUNOSORBENT ASSAY(ELISA) • LATERAL FLOW ASSAY 6. COUNTER IMMUNO-ELECTROPHORESIS (CIE) 7. RADIO IMMUNOSORBENT ASSAY (RIA) • CARBOHYDRATE FERMENTATION TEST: Fermentation tests detect whether yeast can produce gas from the carbohydrate under anaerobic conditions
- 12. Fig. 1 Gram stain of fine needle aspirate of the nodule (1000×) showing intracellular yeast cells with multiple budding inside foamy and debris laden macrophage.
- 13. • SOURCES:https://www.emlab.com/resour ces/fungal-library/sporotrichum-sp/, https://www.medicinenet.com/sporotrichosi s/article.htm#sporotrichosis_facts, https://drfungus.org/knowledge- base/sporotrichum-species/, https://www.Balamuruganr3/serological- tests-in-mycology, Packer ' book, https://www.venkateshk143/fungal- infections-diagnosis
- 14. THANK YOU Any queries ?