CN104745472A - Plant tissue culture tank - Google Patents
Plant tissue culture tank Download PDFInfo
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- CN104745472A CN104745472A CN201510173239.3A CN201510173239A CN104745472A CN 104745472 A CN104745472 A CN 104745472A CN 201510173239 A CN201510173239 A CN 201510173239A CN 104745472 A CN104745472 A CN 104745472A
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- Prior art keywords
- tank
- plant tissue
- circulation tube
- liquid outlet
- culture tank
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a plant tissue culture tank which comprises a culture tank, a circulating device out of the tank, a pneumatic circulating device and a gas distributor, wherein the circulating device out of the tank comprises an additive solution mixer and a culture solution filter; the pneumatic circulating device comprises a culture tank connecting part and a pneumatic mixer; the pneumatic mixer is divided into an upper cavity, a middle narrow part and a lower cavity, and a circulating pipe liquid inlet is formed on the side face of the lower cavity; the circulating device out of the tank can be used for filtering a nutritional liquid and solves the problem that the nutritional liquid which is added is not uniformly mixed, and nutritional components can be added in real time. The pneumatic circulating device powered by introduced compressed air does not increase the shearing force, so that the compressed air and the nutritional liquid are mixed with small and uniform bubbles. Moreover, the additive solution and the nutritional liquid are further mixed, so that the mixing effect is relatively good.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of plant tissue cultivating pot.
Background technology
China is one of country that resources of medicinal plant is the abundantest in the world, and along with a large amount of uses of plant amedica, the excavating and use the speed of growth considerably beyond plant of medicinal plant, resources of medicinal plant is increasingly exhausted.Traditional medicinal plant field production is on the one hand by the impact of " favourable climate and geographical position ", and output and quality fluctuation is large, and pesticide residual contamination problem is serious on the other hand.The bioreactor culture of vegetable cell, tissue and organ has growth rapidly, is easy to the feature controlled, thus can replaces the needs that field production meets market gradually.
Bio-reactor is the reactor for large scale culturing microorganism, plant and animal cell, and the bio-reactor of current culturing plants cell, tissue and organ has arrived the stage of industrialized production.Such as, cultivate Catharanthus Roseus Cell with airlift bioreactor and produce Serpophite [A.H.Scragg et al., Enzyme Microb Technol, 1987,9: 619]; The people such as Korea S S.H.Son carry out reactor large scale culturing to yew cell, adopt the airlift reactor [S.H.Son et al., Plant Cell Reports (2000) 19: 628-633] from 100L to 500L; After several years, Kee-Yoeup Paek adopts spherical bubbling style reactor to carry out large scale culturing to ginseng adventitious root and obtains ginsenoside [the Kee-Yoeup Paek et al. with very strong pharmacologically active, Adv Biochem Engin/Biotechnol (2009) 113: 151-176], obtain the ginseng adventitious root etc. compared with height increament.
Reactor at present for Medicinal Plant Cell, tissue and organ suspension culture mainly contains stirring-type, air lift type, bubbling style etc.From the 80's of eighties of last century, the cultivation of vegetable cell, tissue and organ with reactor just by stirring-type to air lift type and bubbling style transition.Due to vegetable cell, to have volume larger, cell walls is more crisp and have the features such as large vacuole, vegetable cell is very sensitive to shearing force, and its susceptibility is determined by the many factors such as structure of the kind of cell, state, shearforce tolerance, shearing rate and reactor itself.For this feature, the approach of solution, normally from clone, is screened the high yield system of anti-shearing force or is transformed existing clone by cell engineering and genetic engineering means, setting up the strain of anti-shearing force; Transform structure of reactor on this basis, make it have flow field and the good mixing performance of mitigation.
Stirring type bioreactor operating restraint is large, and oxygen delivery capacity is strong, good mixing effect, but the shearing force produced in whipping process is comparatively large, easily cause damage to Vitro Plant tissue, and energy consumption is larger.Comparatively speaking, airlift reactor and bubbling style reactor shearing force less, generally believe that at present bubbling style reactor is the widely applicable bio-reactor of a class both at home and abroad, the constructional feature of this kind of reactor is that bubble rises along liquid phase, contact with liquid phase carry out reacting while agitated liquid to improve rate of mass transfer.
The body of bio-reactor adopts ball-crown body structure and conical structure, overcome the shortcoming that cylindrical airlift bioreactor and Sparged bioreactors easily produce bubble, thus avoid the impact that vegetable cell is subject to volatile matter carbonic acid gas in bubble and so on gas.
For meeting the nutritional need of the whole culturing process of plant tissue, the body of bio-reactor is typically provided with material-feeding port, thief hatch, regularly or irregularly sample in the process of cultivating, supplement nutrient solution again, to measure the Expenditure Levels in dry cell weight, saponin content, polysaccharide content and carbon source, nitrogenous source and phosphorus source.Thus adjustment nutrient solution.To find best nutrient solution.Add initial incubation liquid due to disposable in culturing process, consider the consumption in process of growth, initial incubation liquid is excessive.This excessive nutrient solution, can affect the growth of earlier plant tissue, and early stage plant tissue is fragile, also just further harsh to the requirement of nutrient solution.
Sometimes also need to add Cucumber, to improve the content of active drug composition in culture at specific incubation period.
Because existing bubbling style reactor shearing force is less, mix uneven, Organization individual growth is uneven, also affects output and quality product simultaneously.
Summary of the invention
The object of the invention is for solving stirring reactor shearing force comparatively large, easily damage is caused to Vitro Plant tissue, and bubbling style reactor shearing force is less, mixes uneven problem, and a kind of plant tissue cultivating pot is provided.
A kind of plant tissue cultivating pot, it comprises: culture tank 1, tank external circulating device 2, the pressurized air mouth of pipe 4, gas distributor 5, gas barrier;
Described tank external circulating device 2 comprises: annex solution pipe valve 21, annex solution pipe 22, annex solution mixing tank 23, circulation tube 25, cycle tube valve 27;
Described culture tank 1 is provided with liquid outlet 12 and circulation tube fluid inlet 323;
Described circulation tube fluid inlet 323 is located at below gas distributor 5, and liquid outlet 12 is located at culture tank 1 body middle and upper part, by circulation tube 25, is connected successively with annex solution mixing tank 23, circulation tube fluid inlet 323;
Described annex solution pipe 22 is connected on and adds on liquid mixer 23;
Described tank external circulating device 2 also comprises nutrient solution strainer 24;
Described liquid outlet 12 is multiple, is symmetrical arranged, to ensure that pot liquid is steady;
Described liquid outlet 12 is also provided with liquid outlet filtering net 26;
Described culture tank 1 is provided with a groove 11, and liquid outlet 12 is located on the lower trough rim of groove 11, and liquid outlet 12 opening direction is downward;
Annex solution mixing tank 23 is a cavity, and adding tube liquid 22 is connected on annex solution mixing tank 23; Be arranged in parallel with circulation tube 25;
Air circulation device 3 comprises: culture tank connection section 31, air driven mixer 32; Described air driven mixer 32 is divided into upper chamber 321, middle part narrow 322, lower cavity 324, lower cavity 324 side is provided with circulation tube fluid inlet 323, bottom is provided with the pressurized air mouth of pipe 4, and gas distributor 5 is arranged on above upper chamber, in culture tank connection section 31; Circulation tube fluid inlet 323 is connected with circulation tube 25;
The described pressurized air mouth of pipe 4 is above circulation tube fluid inlet 323.
The invention provides a kind of plant tissue cultivating pot, it comprises: culture tank, tank external circulating device, air circulation device, gas distributor; Tank external circulating device comprises: annex solution mixing tank, nutrient solution strainer; Air circulation device comprises: culture tank connection section, air driven mixer; Described air driven mixer is divided into upper chamber, middle part narrow, lower cavity, and lower cavity side is provided with circulation tube fluid inlet; Nutrition can be added in real time, tank external circulating device can filter by nutritive medium, when solving interpolation nutritive medium, mix uneven problem, air circulation device utilizes the pressurized air passed into be power, does not increase shearing force, achieve pressurized air and nutritive medium gas-liquid mixed, bubble is little, even, and also further mix annex solution and nutritive medium, mixed effect is better simultaneously.
Accompanying drawing explanation
Fig. 1 is the one-piece construction figure of a kind of plant tissue cultivating pot of the present invention;
Fig. 2 is the part sectioned view of the tank external circulating device of a kind of plant tissue cultivating pot of the present invention;
Fig. 3 is the part sectioned view of the air circulation device of a kind of plant tissue cultivating pot of the present invention.
Embodiment
embodiment 1
Refer to Fig. 1-3, a kind of plant tissue cultivating pot, it comprises: culture tank 1, tank external circulating device 2, air circulation device 3, the pressurized air mouth of pipe 4, gas distributor 5, gas barrier;
Tank external circulating device 2 comprises: annex solution pipe valve 21, annex solution pipe 22, annex solution mixing tank 23, nutrient solution strainer 24, circulation tube 25, liquid outlet filtering net 26, cycle tube valve 27;
Described culture tank 1 liquid outlet 12 is located in the middle part of culture tank 1 body, by circulation tube 25, is connected successively with annex solution mixing tank 23 with nutrient solution strainer 24;
Described liquid outlet 12 is also provided with liquid outlet filtering net 26;
Annex solution mixing tank 23 is a cavity, and adding tube liquid 22 is connected on annex solution mixing tank 23; Be arranged in parallel with circulation tube 25;
Air circulation device 3 comprises: culture tank connection section 31, air driven mixer 32; Described air driven mixer 32 is divided into upper chamber 321, middle part narrow 322, lower cavity 324, lower cavity 324 side is provided with circulation tube fluid inlet 323, bottom is provided with the pressurized air mouth of pipe 4, and gas distributor 5 is arranged on above upper chamber, in culture tank connection section 31; Circulation tube fluid inlet 323 is connected with circulation tube 25;
The described pressurized air mouth of pipe 4 is above circulation tube fluid inlet 323;
Described liquid outlet 12 is multiple, is symmetrical arranged, to ensure that pot liquid is steady;
Described culture tank 1 is provided with a groove 11, and liquid outlet 12 is located on the lower trough rim of groove 11, and liquid outlet 12 opening direction is downward.
During use, adjustment nutrient solution is the nutrient solution of the plant tissue growth of the most applicable primary stage of inoculation, close annex solution pipe valve 21, cycle tube valve 27, nutrient solution is added in culture tank 1, inoculation, open air compressor, adjustment air pressure, at this moment culture tank is run by common bubbling style culture tank, can after plant tissue growth be stable, by each time period nutrient consumption level calculation setting of plant tissue, control annex solution addition, open annex solution pipe valve 21, cycle tube valve 27, pressurized air rises, under the effect of pneumatics, nutrient solution filters through liquid outlet filtering net 26, nutrient solution strainer 24 is entered from liquid outlet 12, annex solution mixing tank 23 is entered after filtration, annex solution enters after annex solution mixing tank 23 mixes with nutrient solution from adding tube liquid 22, enter air circulation device 3, gas-liquid mixed, gas distributor 5 redistributes gas-liquid, cultivated material under gravity, concentrate to gas distributor 5, circulate in nutrient solution under the drive of gas-liquid flow, nutrient solution is at tank external circulating device 2 internal recycle simultaneously.Nutrition can be added in real time.The present invention does not add any power, utilize the compressed-air actuated power passed in Sparged bioreactors, employing tank external circulating device filters nutritive medium, annex solution mixes with nutritive medium, air circulation device not only provides power, also achieve gas-liquid mixed, bubble is little, even, and has carried out secondary to annex solution and nutritive medium and mix.
Claims (8)
1. a plant tissue cultivating pot, it comprises: culture tank (1), gas distributor (5), compressed air pipe (4), gas barrier, and it is characterized in that, it also comprises: tank external circulating device (2); Described tank external circulating device (2) comprising: annex solution pipe valve (21), annex solution pipe (22), annex solution mixing tank (23), circulation tube (25), liquid outlet filtering net (26), cycle tube valve (27); Described culture tank (1) is provided with liquid outlet (12) and circulation tube fluid inlet (323); Described circulation tube fluid inlet (323) is located at gas distributor (5) below, and liquid outlet (12) is located at culture tank (1) body middle and upper part, by circulation tube (25), is connected successively with annex solution mixing tank (23), circulation tube fluid inlet (323); Described annex solution pipe (22) is connected on and adds on liquid mixer (23).
2. a kind of plant tissue cultivating pot according to claim 1, is characterized in that: described tank external circulating device (2) also comprises nutrient solution strainer (24).
3. a kind of plant tissue cultivating pot according to claim 1, is characterized in that: described liquid outlet (12), for multiple, is symmetrical arranged, to ensure that pot liquid is steady.
4. a kind of plant tissue cultivating pot according to claim 1 and 2, it is characterized in that: described culture tank (1) is provided with a groove (11), liquid outlet (12) is located on the lower trough rim of groove (11), and liquid outlet 12 opening direction is downward.
5. a kind of plant tissue cultivating pot according to claim 4, is characterized in that: described liquid outlet (12) is also provided with liquid outlet filtering net (26).
6. a kind of plant tissue cultivating pot according to claim 5, is characterized in that: annex solution mixing tank (23) is a cavity, and adding tube liquid (22) and circulation tube (25) be arranged in parallel.
7. a kind of plant tissue cultivating pot according to claim 6, is characterized in that: be also provided with air circulation device (3), comprise: culture tank connection section (31), air driven mixer (32); Described air driven mixer (32) is divided into upper chamber (321), middle part narrow (322), lower cavity (324), lower cavity (324) side is provided with circulation tube fluid inlet (323), bottom is provided with the pressurized air mouth of pipe (4), gas distributor (5) is arranged on above upper chamber, in culture tank connection section (31); Circulation tube fluid inlet (323) is connected with circulation tube (25).
8. a kind of plant tissue cultivating pot according to claim 7, is characterized in that: the described pressurized air mouth of pipe (4) is in circulation tube fluid inlet (323) top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173239.3A CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173239.3A CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
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| CN104745472A true CN104745472A (en) | 2015-07-01 |
| CN104745472B CN104745472B (en) | 2017-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510173239.3A Expired - Fee Related CN104745472B (en) | 2015-04-14 | 2015-04-14 | A kind of plant tissue cultivating pot |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190054393A1 (en) * | 2017-08-18 | 2019-02-21 | Didier Camilleri | Apparatus and Method for Separating Plant Trichomes Using a Double Vortex Turbulence |
| CN112970569A (en) * | 2021-04-02 | 2021-06-18 | 长春工程学院 | Household type aerosol culture method for reducing nitrate of cherry radish |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
Citations (4)
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| US4343231A (en) * | 1980-02-07 | 1982-08-10 | Compagnie Internationale de Participation et d'Investissement "Cipari" S.A. | Brewing apparatus having sampling means delivering suspension to fermenting vessel |
| CN101130744A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte |
| CN201102956Y (en) * | 2007-08-22 | 2008-08-20 | 上海日泰医药设备工程有限公司 | Outer circulation photosynthetic reaction bio-fermentation device |
| US20130164835A1 (en) * | 2011-12-21 | 2013-06-27 | Heliae Development, Llc | Systems and methods for contaminant removal from a microalgae culture |
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2015
- 2015-04-14 CN CN201510173239.3A patent/CN104745472B/en not_active Expired - Fee Related
Patent Citations (4)
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| US4343231A (en) * | 1980-02-07 | 1982-08-10 | Compagnie Internationale de Participation et d'Investissement "Cipari" S.A. | Brewing apparatus having sampling means delivering suspension to fermenting vessel |
| CN101130744A (en) * | 2007-07-26 | 2008-02-27 | 上海交通大学 | Airlift type bioreactor system for continuously bottling and culturing medicinal plant histiocyte |
| CN201102956Y (en) * | 2007-08-22 | 2008-08-20 | 上海日泰医药设备工程有限公司 | Outer circulation photosynthetic reaction bio-fermentation device |
| US20130164835A1 (en) * | 2011-12-21 | 2013-06-27 | Heliae Development, Llc | Systems and methods for contaminant removal from a microalgae culture |
Non-Patent Citations (3)
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| A. G. LIVINGSTON等: "Development of a phenol degrading fluidized bed bioreactor for constant biomass holdup", 《THE CHEMICAL ENGINEERING JOURNAL》 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190054393A1 (en) * | 2017-08-18 | 2019-02-21 | Didier Camilleri | Apparatus and Method for Separating Plant Trichomes Using a Double Vortex Turbulence |
| US10933348B2 (en) | 2017-08-18 | 2021-03-02 | Delta Separations, Llc | Apparatus and method for separating plant trichomes using a double vortex turbulence |
| US10933347B2 (en) * | 2017-08-18 | 2021-03-02 | Delta Separations, Llc | Apparatus and method for separating plant trichomes using a double vortex turbulence |
| CN112970569A (en) * | 2021-04-02 | 2021-06-18 | 长春工程学院 | Household type aerosol culture method for reducing nitrate of cherry radish |
| CN117256478A (en) * | 2023-11-22 | 2023-12-22 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
| CN117256478B (en) * | 2023-11-22 | 2024-02-02 | 上海科缔联科技有限公司 | Continuous fermentation culture system for plant tissues |
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| CN104745472B (en) | 2017-09-19 |
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Granted publication date: 20170919 Termination date: 20210414 |